Document Detail

Ultrasound stimulation of tendon cell proliferation and upregulation of proliferating cell nuclear antigen.
MedLine Citation:
PMID:  16023014     Owner:  NLM     Status:  MEDLINE    
Therapeutic ultrasound has been widely used in sports-related tendon injuries. However, previous research has not examined the molecular mechanism of this therapeutic effect on tendon cell proliferation. This study was designed to determine the in vitro effect of ultrasound on the proliferation of tendon cells intrinsic to rat Achilles tendon. Furthermore, the existence of a correlation between this effect and the expression of the proliferating cell nuclear antigen (PCNA) was also examined. Using cultured tendon cells, cell viability was evaluated by MTT assay. The expression of PCNA protein in cells was evaluated by immunocytochemistry. The mRNA expression of PCNA was determined by reverse transcription-polymerase chain reaction (RT-PCR). A dose-dependent increase in the cellularity of tendon cells by either pulsed or continuous mode ultrasound was demonstrated by MTT assay (p=0.044 for the pulsed mode; p=0.004 for the continuous mode). Ten minutes of treatment achieved maximum cellularity compared to 5 min of treatment time (p=0.010 for pulsed ultrasound; p=0.004 for continuous ultrasound). Immunocyto-chemical staining revealed that ultrasound treated tendon cells were stained more strongly for PCNA than were control cells. Upregulation of PCNA at the mRNA level was also confirmed by RT-PCR. In conclusion, ultrasound stimulates tendon cell proliferation in a process that is probably mediated by the upregulation of PCNA.
Wen-Chung Tsai; Chih-Chin Hsu; Fuk-Tan Tang; Shih-Wei Chou; Ying-Jen Chen; Jong-Hwei S Pang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-26
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  23     ISSN:  0736-0266     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2005-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  970-6     Citation Subset:  IM    
Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Building J, 12F, 5 Fu-Shing Street, Kweishan, Taoyuan 333, Taiwan.
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MeSH Terms
Achilles Tendon / cytology*
Cell Division
Cells, Cultured
Gene Expression
Proliferating Cell Nuclear Antigen / genetics*
Rats, Sprague-Dawley
Tendon Injuries / therapy
Ultrasonic Therapy
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen

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