| Ultrasound-microbubble-mediated NF-kappaB decoy transfection attenuates neointimal formation after arterial injury in mice. | |
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MedLine Citation:
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PMID: 16244495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Decoy transfection is a significant methodology for suppressing gene activation. The decoy can be synthesized easily; however, the major obstacle is the difficulty involved in effective in vivo delivery. METHODS AND RESULTS: We used a fluorescein-labeled decoy to investigate the ultrasound-microbubble-mediated in vivo delivery in normal and injured mouse arteries. We showed that this approach could deliver the decoy into target tissues. In addition, we performed in vivo NF-kappaB decoy transfection into murine injured arteries using the ultrasound-microbubble method. Murine femoral arteries were injured using flexible wires to establish arterial injury. Pathologically, neointima/media areas in the NF-kappaB decoy transfection using ultrasound-microbubble group showed less than those in the control groups. Immunohistochemistry revealed that enhanced expression of inflammatory factors was observed in nontreated injured arteries, while the NF-kappaB decoy suppressed the expression. CONCLUSION: We revealed that ultrasound-microbubble delivery of the decoy is effective for transfection into target organs. We also indicated that NF-kappaB decoy transfection using this method has potential for the suppression of neointimal formation. Ultrasound-mediated transfection of the decoy can be beneficial for the clinical treatment of restenosis after coronary intervention and other cardiovascular diseases. |
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Authors:
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Hiroshi Inagaki; Jun-ichi Suzuki; Masahito Ogawa; Yoshiaki Taniyama; Ryuichi Morishita; Mitsuaki Isobe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-20 |
Journal Detail:
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Title: Journal of vascular research Volume: 43 ISSN: 1018-1172 ISO Abbreviation: J. Vasc. Res. Publication Date: 2006 |
Date Detail:
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Created Date: 2005-12-23 Completed Date: 2006-01-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9206092 Medline TA: J Vasc Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 12-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2006 S. Karger AG, Basel. |
Affiliation:
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Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Femoral Artery / immunology, injuries*, pathology Gene Therapy / methods* Male Mice Mice, Inbred C57BL Microbubbles* NF-kappa B / genetics* Transfection / methods* Tunica Intima / immunology, pathology Ultrasonics* Vasculitis / immunology, pathology |
| Chemical | |
Reg. No./Substance:
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0/NF-kappa B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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