| Ultrasound-enhanced thrombolytic effect of tissue plasminogen activator-loaded echogenic liposomes in an in vivo rabbit aorta thrombus model--brief report. | |
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MedLine Citation:
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PMID: 21441137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Ultrasound enhances thrombolysis when combined with a thrombolytic and a contrast agent. This study aimed to evaluate the thrombolytic effect of our tissue plasminogen activator (tPA)-loaded echogenic liposomes (ELIP) in an in vivo clot model, with and without ultrasound treatment. METHODS AND RESULTS: The femoral arteries of New Zealand White rabbits (n=4 per group) were cannulated. The abdominal aortas were denuded, and thrombi were created using a solution of sodium ricinoleate plus thrombin. Rabbits were then randomly selected to receive tPA-loaded ELIP (200 μg of tPA/5 mg of lipid) or empty ELIP with or without pulsed (color) Doppler ultrasound (5.7 MHz) for 2 minutes. Thrombus was imaged and echogenicity analyzed before and after ELIP injection. Blood flow velocities were measured at baseline, after clot formation, and serially after treatment up to 15 minutes. tPA-loaded ELIP highlighted thrombus in the abdominal aorta more effectively than empty ELIP (P<0.05). Ultrasound enhanced the thrombolytic effect of tPA-loaded ELIP, resulting in earlier and more complete recanalization rates (P<0.001). CONCLUSION: This study demonstrates effective highlighting of clots and thrombolytic effect of tPA-loaded ELIP in an in vivo rabbit aorta clot model. Doppler ultrasound treatment enhances this thrombolytic effect, resulting in earlier and more complete recanalization rates. |
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Authors:
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Susan T Laing; Melanie Moody; Beverly Smulevitz; Hyunggun Kim; Patrick Kee; Shaoling Huang; Christy K Holland; David D McPherson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-03-24 |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 31 ISSN: 1524-4636 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-19 Completed Date: 2011-07-26 Revised Date: 2012-02-29 |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1357-9 Citation Subset: IM |
Affiliation:
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Division of Cardiology, Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX, USA. susan.t.laing@uth.tmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Abdominal Aortic Diseases / therapy* Liposomes Male Rabbits Thrombolytic Therapy / methods* Thrombosis / therapy* Tissue Plasminogen Activator / administration & dosage* Ultrasonic Therapy* |
| Grant Support | |
ID/Acronym/Agency:
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2R01-HL074002/HL/NHLBI NIH HHS; 2R01-NS047603/NS/NINDS NIH HHS; 3R01-HL059586/HL/NHLBI NIH HHS; R01 HL059586-02/HL/NHLBI NIH HHS; R01 HL059586-03/HL/NHLBI NIH HHS; R01 HL059586-04A2/HL/NHLBI NIH HHS; R01 HL059586-05/HL/NHLBI NIH HHS; R01 HL059586-06/HL/NHLBI NIH HHS; R01 HL059586-07/HL/NHLBI NIH HHS; R01 HL059586-08A1/HL/NHLBI NIH HHS; R01 HL059586-08A1W1/HL/NHLBI NIH HHS; R01 HL059586-09/HL/NHLBI NIH HHS; R01 HL059586-10/HL/NHLBI NIH HHS; R01 HL059586-11/HL/NHLBI NIH HHS; R01 HL074002-01A1/HL/NHLBI NIH HHS; R01 HL074002-02/HL/NHLBI NIH HHS; R01 HL074002-03/HL/NHLBI NIH HHS; R01 HL074002-04/HL/NHLBI NIH HHS; R01 HL074002-05A2/HL/NHLBI NIH HHS; R01 HL074002-06/HL/NHLBI NIH HHS; R01 HL074002-07/HL/NHLBI NIH HHS; R01 NS047603-02/NS/NINDS NIH HHS; R56 HL059586-08/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; EC 3.4.21.68/Tissue Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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