Document Detail

Ultrasonographic and histological characterization of the placenta of somatic nuclear transfer-derived pregnancies in dairy cattle.
MedLine Citation:
PMID:  17983647     Owner:  NLM     Status:  MEDLINE    
The high incidence of pregnancy loss and prenatal morbidity and mortality in cloned animals may be due to placental insufficiency, thereby compromising fetal survival. Our objective was to characterize morphological changes in fetal membranes of cloned bovine pregnancies. Two groups of cows with cloned fetuses, produced by two cloning techniques, a commercial group (n=16) and a hand-made group (n=4), and control fetuses derived from traditional embryo transfer (n=6) or AI (n=6), were compared at various stages of gestation (Days 80, 120, 150, 180, 210, and 240; Day 0=estrus). Thickness and shape of the amniotic membrane, placentome shape and length, umbilical cord shape and diameter, and fetal fluid echodensities were assessed by ultrasonography, and the placenta was evaluated histologically. Only eight (40%) of cloned pregnancies reached term and seven calves (35%) were alive at birth. Both placentome length and umbilical cord diameter were larger (P<0.05) in clones than in normal fetuses at all stages of gestation. Amniotic membrane abnormalities (Day 120) including focal edema and the presence of a series of nodules were detected in 38% of the clones and were always accompanied by hyper-echodense spikes or irregularities (detected ultrasonographically) around the umbilical cord. Histopathology revealed degenerate inflammatory cells, edematous chorioallantoic membranes, and decreased epithelial thickness. We inferred that these morphological anomalies of placentomes compromised fetal development, and we concluded that ultrasonographic monitoring of pregnancies enabled characterization of changes in the placentae and may be useful to assess fetal well-being.
H R Kohan-Ghadr; R C Lefebvre; G Fecteau; L C Smith; B D Murphy; J Suzuki Junior; C Girard; P Hélie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-05
Journal Detail:
Title:  Theriogenology     Volume:  69     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-28     Completed Date:  2008-03-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-30     Citation Subset:  IM    
Centre de Recherche en Reproduction Animale, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, Québec, Canada.
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MeSH Terms
Amnion / pathology,  physiology,  ultrasonography
Animals, Newborn
Cattle / physiology*
Cloning, Organism
Fetal Development / physiology*
Histocytochemistry / veterinary
Longitudinal Studies
Nuclear Transfer Techniques / veterinary*
Placenta / pathology,  physiology*,  ultrasonography
Ultrasonography, Prenatal / veterinary
Umbilical Cord / pathology,  physiology*,  ultrasonography

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