Document Detail


Ultrasonographic evidence of phenotypic instability during hepatocarcinogenesis in N-nitrosomorpholine-treated rats.
MedLine Citation:
PMID:  12127056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carcinogen-induced hepatoma in immunocompetent animal models has shown a progress similar to the clinical course of human hepatoma. Ultrasonography (US) was used for consecutive evaluation of the phenotypic changes in Sprague-Dawley rats exposed for 8 weeks to N-nitrosomorpholine (NNM, 200 mg/L). Three distinctive US findings were ascites, coarseness (defined as small and heterogeneously widespread increased echogenecity), and nodularity (defined as a >0.6-cm-sized echogenic region and clearly showing a tumor-like mass). Abdominal ascites was observed in 5 of 26 rats at week 8 NNM posttreatment and the number of rats showing ascites gradually increased. Coarseness (22 of 26 rats) and nodularity (1 of 18) appeared at weeks 8 and 17 NNM posttreatment, respectively. The gross and histological findings indicated that coarseness and nodularity shown in US reflected fibrosis and hepatocellular carcinoma or cholangiofibroma, respectively. The computer-aided quantification of coarseness and nodularity showed that the regression-linked phenotypic instability was present in coarseness but not in nodularity. We conclude that the heterogeneity of preneoplasia in NNM-treated rats might be induced by phenotypic instability rather than random initiating events of preneoplastic lesion.
Authors:
Jong-Young Oh; Jin-Sook Jeong; Yu-jin Kim; Kyoung-Jin Nam; Byung-Ho Park; Eun-Young Kwon; Young-Hoon Kim; Tae-Ho Hwang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  73     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-19     Completed Date:  2002-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Affiliation:
Institute of Medical Science, Department of Radiology, Medical College of Dong-A University, Busan 602-714, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascites / complications,  ultrasonography
Carcinoma, Hepatocellular / chemically induced*,  complications,  ultrasonography*
Disease Models, Animal
Fibrosis / chemically induced,  complications,  ultrasonography
Image Processing, Computer-Assisted
Liver / drug effects,  pathology,  ultrasonography*
Liver Neoplasms, Experimental / chemically induced*,  complications,  ultrasonography*
Male
Nitrosamines*
Phenotype
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Nitrosamines; 59-89-2/N-nitrosomorpholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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