Document Detail


Ultrasonographic fetal sex identification in pregnant sheep derived from natural mating and embryo transfer.
MedLine Citation:
PMID:  20228614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to identify the migration period of the genital tubercle and the period of visualization of external genital structures in fetuses of the Dorper breed of sheep derived from natural mating and from fresh, frozen and vitrified embryo transfer. Transrectal ultrasound was performed using a double-frequency linear transducer (6.0 and 8.0 MHz) to monitor 130 ewe fetuses distributed in the four treatments regarding embryo origin. The accuracy of the ultrasound was 100% in this experiment. The fetuses originated from controlled natural mating (NM) and from fresh (FrE), frozen (FE) and vitrified (VE) embryo transfer, with embryos collected 7 days after breeding. Migration of the genital tubercle occurred earlier (P<0.05) in NM (42.21+/-2.86 days) than in FrE (43.98+/-3.00 days), FE (44.97+/-1.83 days) and VE (44.58+/-1.97 days). Visualization of the scrotal bag, prepuce and vulva occurred, respectively, earlier (P<0.05) in NM (45.22+/-1.25, 45.95+/-1.53 and 45.01+/-1.03 days) than in FrE (48.91+/-1.92, 48.52+/-1.41 and 47.41+/-1.41 days), FE (49.97+/-1.08, 49.18+/-2.00 and 47.64+/-1.82 days) and VE (50.12+/-1.66, 49.27+/-1.61 and 47.93+/-1.92 days). The results show that fetal sexing can be accomplished from the 50th day onward in fetuses produced by natural mating and from the 55th day onward in fetuses derived from fresh, frozen and vitrified embryos. It can also be concluded that real-time ultrasonography is a reliable tool for fetal sex determination in sheep taking into account both the location of the genital tubercle and the identification of external genital structures.
Authors:
Leopoldo Mayer de Freitas Neto; Maico Henrique Barbosa dos Santos; Cristiano Rocha de Aguiar Filho; José Monteiro de Almeida Irmão; Eduardo Luiz Cavalcanti Caldas; Jairo Pereira Neves; Paulo Fernandes Lima; Marcos Antonio Lemos de Oliveira
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Publication Detail:
Type:  Journal Article     Date:  2010-03-15
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  56     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-02     Completed Date:  2010-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  347-50     Citation Subset:  IM    
Affiliation:
Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cloning, Organism / methods*
Embryo Transfer / methods*,  veterinary*
Female
Pregnancy
Pregnancy, Animal
Reproducibility of Results
Reproductive Techniques, Assisted
Sex Determination (Analysis) / methods*
Sheep
Sheep, Domestic / embryology*
Ultrasonography / methods
Ultrasonography, Prenatal / methods*,  veterinary*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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