| Ultrasonographic fetal sex identification in pregnant sheep derived from natural mating and embryo transfer. | |
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MedLine Citation:
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PMID: 20228614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to identify the migration period of the genital tubercle and the period of visualization of external genital structures in fetuses of the Dorper breed of sheep derived from natural mating and from fresh, frozen and vitrified embryo transfer. Transrectal ultrasound was performed using a double-frequency linear transducer (6.0 and 8.0 MHz) to monitor 130 ewe fetuses distributed in the four treatments regarding embryo origin. The accuracy of the ultrasound was 100% in this experiment. The fetuses originated from controlled natural mating (NM) and from fresh (FrE), frozen (FE) and vitrified (VE) embryo transfer, with embryos collected 7 days after breeding. Migration of the genital tubercle occurred earlier (P<0.05) in NM (42.21+/-2.86 days) than in FrE (43.98+/-3.00 days), FE (44.97+/-1.83 days) and VE (44.58+/-1.97 days). Visualization of the scrotal bag, prepuce and vulva occurred, respectively, earlier (P<0.05) in NM (45.22+/-1.25, 45.95+/-1.53 and 45.01+/-1.03 days) than in FrE (48.91+/-1.92, 48.52+/-1.41 and 47.41+/-1.41 days), FE (49.97+/-1.08, 49.18+/-2.00 and 47.64+/-1.82 days) and VE (50.12+/-1.66, 49.27+/-1.61 and 47.93+/-1.92 days). The results show that fetal sexing can be accomplished from the 50th day onward in fetuses produced by natural mating and from the 55th day onward in fetuses derived from fresh, frozen and vitrified embryos. It can also be concluded that real-time ultrasonography is a reliable tool for fetal sex determination in sheep taking into account both the location of the genital tubercle and the identification of external genital structures. |
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Authors:
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Leopoldo Mayer de Freitas Neto; Maico Henrique Barbosa dos Santos; Cristiano Rocha de Aguiar Filho; José Monteiro de Almeida Irmão; Eduardo Luiz Cavalcanti Caldas; Jairo Pereira Neves; Paulo Fernandes Lima; Marcos Antonio Lemos de Oliveira |
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Publication Detail:
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Type: Journal Article Date: 2010-03-15 |
Journal Detail:
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Title: The Journal of reproduction and development Volume: 56 ISSN: 0916-8818 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-10-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
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Languages: eng Pagination: 347-50 Citation Subset: IM |
Affiliation:
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Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cloning, Organism / methods* Embryo Transfer / methods*, veterinary* Female Pregnancy Pregnancy, Animal Reproducibility of Results Reproductive Techniques, Assisted Sex Determination (Analysis) / methods* Sheep Sheep, Domestic / embryology* Ultrasonography / methods Ultrasonography, Prenatal / methods*, veterinary* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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