Document Detail

Ultrasonically induced cell damage and active oxygen generation by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7-diaspartic acid: on the mechanism of sonodynamic activation.
MedLine Citation:
PMID:  12595087     Owner:  NLM     Status:  MEDLINE    
Ultrasonically induced cell damage and active oxygen generation with 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7-diaspartic acid (ATX-S10) were compared in the same in vitro insonation setup. Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of ATX-S10. The viability was determined by Trypan blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of ATX-S10 in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR). Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 40-160 microM ATX-S10. Both rates correlated very well. The enhancement of both rates with ATX-S10 was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of ATX-S10.
Nagahiko Yumita; Isao Sakata; Susumu Nakajima; Shin-ichiro Umemura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1620     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-21     Completed Date:  2003-07-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
School of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
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MeSH Terms
Electron Spin Resonance Spectroscopy
Free Radical Scavengers / chemistry
Histidine / chemistry
Molecular Structure
Nitrogen Oxides / analysis,  chemical synthesis
Porphyrins* / antagonists & inhibitors
Reactive Oxygen Species / analysis,  chemistry*
Sarcoma 180
Tumor Cells, Cultured
Reg. No./Substance:
0/ATX-S10; 0/Free Radical Scavengers; 0/Nitrogen Oxides; 0/Porphyrins; 0/Reactive Oxygen Species; 14332-28-6/nitroxyl; 71-00-1/Histidine

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