Document Detail


Ultrasonic Doppler measurements of blood flow velocity of rabbit retinal vessels using a 45-MHz needle transducer.
MedLine Citation:
PMID:  20162299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The purpose of this study is to measure blood flow velocity of rabbit retinal vessels using a 45-MHz ultrasonic Doppler system with a needle transducer.
METHODS: A high-frequency pulsed Doppler system that utilizes a 45-MHz PMN-PT needle transducer was developed to measure retinal blood flow velocity in situ. The pulsed Doppler allowed the differentiation of retinal from choroidal blood flow velocity. The needle transducer was inserted into the vitreous cavity through a 20-gauge incision port to access the retinal vessels. The first phase of the experiment evaluated the reproducibility of the measurements. The second phase measured velocities at four positions from the optic disc edge to the distal part of each vessel in nine eyes for the temporal and six eyes for the nasal portions. The angle between the transducer and the retinal vessel at each site was measured in enucleated rabbit eyes to estimate and compensate for measurement errors.
RESULTS: In the first phase, the average measurement error was 5.97 +/- 1.34%. There was no significant difference comparing all eyes. In the second phase, the velocities gradually slowed from the disc edge to the distal part, and temporal velocities were faster than nasal velocities at all measurement sites.
CONCLUSION: This study demonstrated the feasibility of reliably measuring retinal blood flow velocity using a 45-MHz ultrasonic Doppler system with a needle transducer.
Authors:
Naoki Matsuoka; Dong-Guk Paeng; Ruimin Chen; Hossein Ameri; Walid Abdallah; Qifa Zhou; Amani Fawzi; K K Shung; Mark Humayun
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-02-17
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  248     ISSN:  1435-702X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-06-23     Revised Date:  2011-06-10    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  675-80     Citation Subset:  IM    
Affiliation:
Doheny Retina Institute, Los Angeles, CA, USA, naomat2@hotmail.com.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity / physiology*
Feasibility Studies
Rabbits
Regional Blood Flow
Reproducibility of Results
Retinal Vessels / physiology*
Transducers*
Ultrasonography, Doppler, Pulsed / instrumentation*
Grant Support
ID/Acronym/Agency:
EY03040/EY/NEI NIH HHS; P41 EB002182-13/EB/NIBIB NIH HHS
Comments/Corrections

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