| Ultrashort partial-filling technique in capillary electrophoresis for infinite resolution of tramadol enantiomers and its metabolites with highly sulfated cyclodextrins. | |
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MedLine Citation:
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PMID: 15352008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The possibility to enhance resolution to infinite value in chiral capillary electrophoresis is attained as soon as the apparent mobility of one enantiomer becomes opposite to the other. This could be achieved on the basis of the carrier ability of multiple charged chiral selectors such as highly sulfated cyclodextrin (HS-CD). With tramadol and its phase I metabolites selected as model compounds, the HS-gamma-CD was found to be the most appropriate chiral selector. The CD concentration was determined where one enantiomer still migrated as a cation while the other migrated in the opposite side. Besides the chiral selector concentration, secondary parameters such as buffer concentration appeared to be critical to reach infinite resolution. The latter was achieved with partial filling technique using ultrashort separation zones (a few mm). In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. The results obtained point to the prevailing role of complex mobility differences in the enantioselectivity mechanism. |
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Authors:
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Serge Rudaz; Thomas Le Saux; Josiane Prat; Pierre Gareil; Jean-Luc Veuthey |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Electrophoresis Volume: 25 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-09-07 Completed Date: 2005-02-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 2761-71 Citation Subset: IM |
Affiliation:
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Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmacy, University of Geneva, Geneva, Switzerland. serge.rudaz@pharm.unige.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Buffers Cyclodextrins / chemistry Electrophoresis, Capillary / methods* Molecular Structure Osmolar Concentration Stereoisomerism Temperature Tramadol / chemistry*, isolation & purification*, metabolism Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Cyclodextrins; 27203-92-5/Tramadol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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