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Ultrasensitive electrochemiluminescence immunosensor using PtAg@carbon nanocrystals composites as labels and carbon nanotubes-chitosan/gold nanoparticles as enhancer.
MedLine Citation:
PMID:  22428155     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An ultrasensitive electrochemiluminescence (ECL) immunosensor was developed using PtAg@carbon nanocrystals (CNCs) as excellent labels based on carbon nanotubes-chitosan/AuNPs (CNT-CHIT/AuNPs) composite modified screen-printed carbon electrodes (SPCEs) for prostate protein antigen (PSA) detection. The CNCs were obtained simply by electro-oxidation of graphite with abundant carboxyl groups at their surfaces. The PtAg bimetallic nanocomposites with hierarchically hollow structures were fabricated through simple replacement reaction using dealloyed nanoporous silver (NPS) as both a template and reducing agent. Structure characterization was obtained by means of transmission electron microscope (TEM) and scanning electron microscope (SEM) images. The PtAg@CNCs composites exhibit a 6 times higher ECL intensity than the pure CNCs labeled anti-PSA. The as-prepared CNT-CHIT/AuNPs composite can attach more antibody than pure CNTs. Due to the dual-amplification techniques, the concentrations of PSA were obtained in the range from 1 pg mL(-1) to 50 ng mL(-1) with a detection limit of 0.6 pg mL(-1). Finally, the as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection.
Authors:
Meng Zhang; Weijian Dai; Mei Yan; Shenguang Ge; Jinghua Yu; Xianrang Song; Wei Xu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-19
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. ujn.yujh@gmail.com.
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