| Ultrasensitive determination of serum albumin using resonance light scattering based on ZnS-polyacrylic acid nanoparticles. | |
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MedLine Citation:
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PMID: 20211579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ZnS-polyacrylic acid (ZnS-PAA) was prepared by an in situ polymerization method using nano-ZnS as core in the presence of acrylic acid (AA), and ZnS-PAA nanoparticles was characterized by ultraviolet spectrometry (UV) and transmission electron microscopy (TEM). Based on the significant increase of the resonance light scattering (RLS) intensity with the interaction between nanoparticles and serum albumin, RLS method was developed for the sensitive determination of serum albumin (BSA and HSA). Under optimum conditions, the change of the intensity (DeltaI) of the RLS spectra at lambda=392nm was linearly proportional to the concentration of BSA and HSA. The linear range was 1-100ngmL(-1) for HSA and 1-120ngmL(-1) for BSA, and the limit of detection (LOD) was 0.4ngmL(-1) for HSA and 0.5ngmL(-1) for BSA. This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances. |
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Authors:
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Li Peng; Chunya Li; Hongbo Xue; Guoqing Zhan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-11 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 75 ISSN: 1873-3557 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-02 Completed Date: 2010-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 1497-500 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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chemistry* Calibration Humans Hydrogen-Ion Concentration Indicators and Reagents Light* Limit of Detection Nanoparticles / chemistry*, ultrastructure Scattering, Radiation* Serum Albumin / analysis*, ultrastructure Spectrophotometry, Ultraviolet / methods* Sulfides / chemistry* Time Factors Zinc Compounds / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Indicators and Reagents; 0/Serum Albumin; 0/Sulfides; 0/Zinc Compounds; 1314-98-3/zinc sulfide; 9003-01-4/carbopol 940 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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