Document Detail

Ultrasensitive determination of serum albumin using resonance light scattering based on ZnS-polyacrylic acid nanoparticles.
MedLine Citation:
PMID:  20211579     Owner:  NLM     Status:  MEDLINE    
ZnS-polyacrylic acid (ZnS-PAA) was prepared by an in situ polymerization method using nano-ZnS as core in the presence of acrylic acid (AA), and ZnS-PAA nanoparticles was characterized by ultraviolet spectrometry (UV) and transmission electron microscopy (TEM). Based on the significant increase of the resonance light scattering (RLS) intensity with the interaction between nanoparticles and serum albumin, RLS method was developed for the sensitive determination of serum albumin (BSA and HSA). Under optimum conditions, the change of the intensity (DeltaI) of the RLS spectra at lambda=392nm was linearly proportional to the concentration of BSA and HSA. The linear range was 1-100ngmL(-1) for HSA and 1-120ngmL(-1) for BSA, and the limit of detection (LOD) was 0.4ngmL(-1) for HSA and 0.5ngmL(-1) for BSA. This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances.
Li Peng; Chunya Li; Hongbo Xue; Guoqing Zhan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-11
Journal Detail:
Title:  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy     Volume:  75     ISSN:  1873-3557     ISO Abbreviation:  Spectrochim Acta A Mol Biomol Spectrosc     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-02     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9602533     Medline TA:  Spectrochim Acta A Mol Biomol Spectrosc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1497-500     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
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MeSH Terms
Acrylic Resins / chemistry*
Hydrogen-Ion Concentration
Indicators and Reagents
Limit of Detection
Nanoparticles / chemistry*,  ultrastructure
Scattering, Radiation*
Serum Albumin / analysis*,  ultrastructure
Spectrophotometry, Ultraviolet / methods*
Sulfides / chemistry*
Time Factors
Zinc Compounds / chemistry*
Reg. No./Substance:
0/Acrylic Resins; 0/Indicators and Reagents; 0/Serum Albumin; 0/Sulfides; 0/Zinc Compounds; 1314-98-3/zinc sulfide; 9003-01-4/carbopol 940

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