Document Detail

Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes.
MedLine Citation:
PMID:  18973779     Owner:  NLM     Status:  MEDLINE    
The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments.
B Geusens; J Lambert; S C De Smedt; K Buyens; N N Sanders; M Van Gele
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-17
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  133     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-03-05     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  214-20     Citation Subset:  IM    
Department of Dermatology, Ghent University Hospital, Belgium.
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MeSH Terms
Cations / chemistry
Cell Survival
Cells, Cultured
Cryoelectron Microscopy
Drug Stability
Fatty Acids, Monounsaturated / chemistry
Gene Expression
Infant, Newborn
Liposomes / chemistry*
Melanocytes / cytology,  metabolism*
Micropore Filters
Myosin Heavy Chains / genetics,  metabolism
Myosin Type V / genetics,  metabolism
Particle Size
Quaternary Ammonium Compounds / chemistry
RNA Interference
RNA, Small Interfering / chemistry,  genetics*,  ultrastructure
Sodium Cholate / chemistry
Transfection / methods*
Reg. No./Substance:
0/Cations; 0/Fatty Acids, Monounsaturated; 0/Liposomes; 0/Myosin Heavy Chains; 0/Quaternary Ammonium Compounds; 0/RNA, Small Interfering; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 148971-15-7/MYO5A protein, human; 361-09-1/Sodium Cholate; EC 3.6.1.-/Myosin Type V

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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