| Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes. | |
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MedLine Citation:
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PMID: 18973779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments. |
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Authors:
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B Geusens; J Lambert; S C De Smedt; K Buyens; N N Sanders; M Van Gele |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-17 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: 133 ISSN: 1873-4995 ISO Abbreviation: J Control Release Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-27 Completed Date: 2009-03-05 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: Netherlands |
Other Details:
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Languages: eng Pagination: 214-20 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Ghent University Hospital, Belgium. Barbara.Geusens@Ugent.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cations
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chemistry Cell Survival Cells, Cultured Cryoelectron Microscopy Drug Stability Electricity Fatty Acids, Monounsaturated / chemistry Gene Expression Humans Infant, Newborn Liposomes / chemistry* Male Melanocytes / cytology, metabolism* Micropore Filters Myosin Heavy Chains / genetics, metabolism Myosin Type V / genetics, metabolism Particle Size Quaternary Ammonium Compounds / chemistry RNA Interference RNA, Small Interfering / chemistry, genetics*, ultrastructure Sodium Cholate / chemistry Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Fatty Acids, Monounsaturated; 0/Liposomes; 0/Myosin Heavy Chains; 0/Quaternary Ammonium Compounds; 0/RNA, Small Interfering; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 148971-15-7/MYO5A protein, human; 361-09-1/Sodium Cholate; EC 3.6.1.-/Myosin Type V |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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