Document Detail


Ultracytochemical localization of glucose-6-phosphatase in Chang rat hepatoma in vivo and in vitro.
MedLine Citation:
PMID:  192899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The standard lead precipitation method was used for ultracytochemical localization of glucose-6-phosphatase (G-6-Pase) in the in vivo and in vitro forms of the Chang rat hepatoma and in the normal adult rat liver. Reaction product was visualized as very fine particulate within the cisternae of the nuclear envelope and endoplasmic reticulum. Cytochemically, the amount of the G-6-Pase reaction product in both forms of the tumor cells was obviously less than that in the normal hepatocytes. Apparently, the enzyme was not completely deleted from the hepatoma cells. The results supported some biochemical data of certain other hepatomas. The successful ultracytochemical localization of G-6-Pase in cultured hepatoma cells has not been reported previously.
Authors:
P C Moller; M Yokoyama; J P Chang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the National Cancer Institute     Volume:  58     ISSN:  0027-8874     ISO Abbreviation:  J. Natl. Cancer Inst.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503089     Medline TA:  J Natl Cancer Inst     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1401-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular / enzymology*,  ultrastructure
Cells, Cultured
Endoplasmic Reticulum / enzymology
Glucose-6-Phosphatase / metabolism*
Liver / enzymology
Liver Neoplasms / enzymology*,  ultrastructure
Neoplasms, Experimental / enzymology,  ultrastructure
Nuclear Envelope / enzymology
Rats
Chemical
Reg. No./Substance:
EC 3.1.3.9/Glucose-6-Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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