Document Detail


An Ultrabithorax protein binds sequences near its own and the Antennapedia P1 promoters.
MedLine Citation:
PMID:  2904838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The homeotic gene Ultrabithorax (Ubx), located in the bithorax complex of Drosophila, encodes a family of closely related proteins that direct the developmental fates of posterior thoracic and anterior abdominal metameres. We have purified a member of the Ubx protein family from an overproducing E. coli strain and have shown that it is sequence-specific DNA binding protein. The protein binds tightly to sequences near its own promoter and near the P1 promoter of Antenna-pedia (Antp), a homeotic gene Ubx is known to repress from genetic studies. The binding sites occur in clusters downstream of the transcription start sites, and far upstream at Antp P1. They range in size from 40 to 90 bp, and contain tandem repeats of the trinucleotide TAA or the related hexanucleotide TAA-TCG. These results suggest that the regulatory activities of Ubx are direct and are mediated by binding of Ubx proteins to promoter region sequences.
Authors:
P A Beachy; M A Krasnow; E R Gavis; D S Hogness
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  55     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-02-03     Completed Date:  1989-02-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1069-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Stanford University School of Medicine, California 94305.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites
DNA / metabolism
DNA-Binding Proteins / genetics,  metabolism*
Drosophila / genetics
Escherichia coli
Genes, Homeobox*
Insect Hormones / genetics,  metabolism*
Molecular Sequence Data
Promoter Regions, Genetic*
Repetitive Sequences, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Insect Hormones; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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