| Ultra-trace determination of S-nitrosothiols in blood samples by spectrofluorimetry with 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene. | |
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MedLine Citation:
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PMID: 17531528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Increasing evidence suggests that S-nitrosothiols (RSNO) may represent naturally occurring nitric oxide (NO) surrogates and function as intermediates in NO metabolism. In this work, a simple, sensitive, and selective micromethod is developed and validated for quantification of RSNO. A fluorescent probe 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (DABODIPY) is firstly used to label RSNO. The derivatization reaction is performed in aqueous medium at 30 degrees C for 15min in the presence of 6x10(-5)molL(-1)Hg2+ and the derivative is detected by fluorescence at lambda(ex)/lambda(em)=500/510nm. A linear function of concentration in the range of (2.0-600.0)x10(-8)molL(-1) is observed with a correlation coefficient of 0.9992 and detection limit of 1.2x10(-9)molL(-1) (S/N=3). This technique has been successfully applied to quantify RSNO in some human blood samples including healthy persons and patients suffering from cardiovascular diseases. |
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Authors:
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Ke-Jing Huang; Chun-Xuan Xu; Wan-Zhen Xie; Hua-Shan Zhang; Hong Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-24 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 69 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-04 Completed Date: 2008-05-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 437-42 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Wuhan University, Wuhan 430072, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Boron Compounds*
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chemistry Glutathione / analogs & derivatives, chemistry Heterocyclic Compounds, 3-Ring* / chemistry Humans Ions / chemistry Molecular Structure Nitro Compounds / chemistry S-Nitrosothiols / blood*, chemistry Spectrometry, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/8-(3',4'-diaminophenyl)-difluoroboradiaza-S-indacence; 0/Boron Compounds; 0/Heterocyclic Compounds, 3-Ring; 0/Ions; 0/Nitro Compounds; 0/S-Nitrosothiols; 0/S-nitroglutathione; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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