Document Detail


Ultra-structural identification of interstitial cells of Cajal in the zebrafish Danio rerio.
MedLine Citation:
PMID:  22628160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interstitial cells of Cajal (ICCs) are important mediators of gastrointestinal (GI) motility because of their role as pacemakers in the GI tract. In addition to their function, ICCs are also structurally distinct cells most easily identified by their ultra-structural features and expression of the tyrosine kinase receptor c-KIT. ICCs have been described in mammals, rodents, birds, reptiles, and amphibians, but there are no reports at the ultra-structural level of ICCs within the GI tract of an organism from the teleost lineage. We describe the presence of cells in the muscularis of the zebrafish intestine; these cells have similar features to ICCs in other vertebrates. The ICC-like cells are associated with the muscularis, are more electron-dense than surrounding smooth muscle cells, possess long cytoplasmic processes and mitochondria, and are situated opposing enteric nervous structures. In addition, immunofluorescent and immunoelectron-microscopic studies with antibodies targeting the zebrafish ortholog of a putative ICC marker, c-KIT (kita), showed c-kit immunoreactivity in zebrafish ICCs. Taken together, these data represent the first ultra-structural characterization of cells in the muscularis of the zebrafish Danio rerio and suggest that ICC differentiation in vertebrate evolution dates back to the teleost lineage.
Authors:
Evan R Ball; Miho M Matsuda; Louis Dye; Victoria Hoffmann; Patricia M Zerfas; Eva Szarek; Adam Rich; Ajay B Chitnis; Constantine A Stratakis
Publication Detail:
Type:  Journal Article     Date:  2012-05-25
Journal Detail:
Title:  Cell and tissue research     Volume:  349     ISSN:  1432-0878     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-09     Completed Date:  2012-12-10     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  483-91     Citation Subset:  IM    
Affiliation:
Section on Endocrinology & Genetics, Program on Developmental Endocrinology & Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Building 10, CRC, Room 1-3330, 10 Center Drive, MSC1103, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Interstitial Cells of Cajal / ultrastructure*
Intestines / cytology,  ultrastructure*
Microscopy, Electron
Proto-Oncogene Proteins c-kit / analysis
Zebrafish / anatomy & histology*
Grant Support
ID/Acronym/Agency:
ZIA HD008920-01/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.10.1/Proto-Oncogene Proteins c-kit
Comments/Corrections

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