Document Detail


Ultra-structural changes and developmental potential of porcine oocytes following vitrification.
MedLine Citation:
PMID:  16895747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study evaluated the effects of exposure and/or vitrification of porcine metaphase II (MII) oocytes on their in vitro viability and ultra-structural changes with two experiments. Experiment 1 examined the effect of vitrified oocytes on microtubule localization, mitochondrial morphology, chromosome organization and the developmental rate in IVF control and vitrified oocytes. Oocytes matured for 44 h were subjected to IVF (IVF control). Oocytes matured for 42 h were exposed to cryoprotectants (CPA control), followed by 2h culture, and subjected to IVF. Oocytes vitrified at 42 h post-maturation were warmed, cultured for 2h, and subjected to IVF (vitrified). Experiment 2 evaluated the effect of oocytes freezing on development of ICSI with and without activation and parthenotes. Fresh and vitrified oocytes were subjected to ICSI with and without electrical activation. Cleavage and blastocyst rates were significantly (P<0.05) lower in vitrified IVF, parthenote and ICSI embryos than those in fresh counterparts. Between ICSI embryos from fresh oocytes and vitrified oocytes, the rates of blastocyst were significantly higher (P<0.05) in activated group than the group without activation. Significant differences (P<0.05) were observed in normal spindle configuration of vitrified (43.5%) compared to control (81.0%) oocytes, but no significant difference was observed between CPA exposed and control groups. In conclusion, porcine oocytes at MII stage are very sensitive to vitrification with altered microtubule localization and mitochondrial organization thus resulting in impaired fertilization and embryo development.
Authors:
Lian-Yu Shi; Hai-Feng Jin; Jung-Gon Kim; B Mohana Kumar; S Balasubramanian; Sang-Young Choe; Gyu-Jin Rho
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-08
Journal Detail:
Title:  Animal reproduction science     Volume:  100     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-04     Completed Date:  2007-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  128-40     Citation Subset:  IM    
Affiliation:
College of Veterinary Medicine, Gyeongsang National University, Chinju 660-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes / physiology
Cryopreservation / methods,  veterinary*
Embryo Culture Techniques / veterinary*
Fertilization in Vitro / veterinary*
Metaphase
Mitochondria / physiology
Oocytes / cytology,  growth & development*,  ultrastructure*
Sperm Injections, Intracytoplasmic / veterinary
Swine*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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