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Ultra-small gadolinium oxide nanoparticles to image brain cancer cells in vivo with MRI.
MedLine Citation:
PMID:  21125569     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The majority of contrast agents used in magnetic resonance imaging (MRI) is based on the rare-earth element gadolinium. Gadolinium-based nanoparticles could find promising applications in pre-clinical diagnostic procedures of certain types of cancer, such as glioblastoma multiforme. This is one of the most malignant, lethal and poorly accessible forms of cancer. Recent advances in colloidal nanocrystal synthesis have led to the development of ultra-small crystals of gadolinium oxide (US-Gd(2)O(3), 2-3 nm diameter). As of today, this is the smallest and the densest of all Gd-containing nanoparticles. Cancer cells labeled with a sufficient quantity of this compound appear bright in T(1)-weighted MRI images. Here we demonstrate that US-Gd(2)O(3) can be used to label GL-261 glioblastoma multiforme cells, followed by localization and visualization in vivo using MRI. Very high amounts of Gd are efficiently internalized and retained in cells, as confirmed with TEM and ICP-MS. Labeled cells were visualized in vivo at 1.5 T using the chicken embryo model. This is one more step toward the development of "positively contrasted" cell tracking procedures with MRI. Copyright © 2010 John Wiley & Sons, Ltd.
Authors:
Luc Faucher; Andrée-Anne Guay-Bégin; Jean Lagueux; Marie-France Côté; Eric Petitclerc; Marc-André Fortin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-1
Journal Detail:
Title:  Contrast media & molecular imaging     Volume:  -     ISSN:  1555-4317     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101286760     Medline TA:  Contrast Media Mol Imaging     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Axe Métabolisme, santé vasculaire et rénale, Centre hospitalier universitaire de Québec, 10 rue de l'Espinay Québec, Canada G1L 3L5.
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