Document Detail

Ultra-performance liquid chromatographic analysis of amino acids in protein hydrolysates using an automated pre-column derivatisation method.
MedLine Citation:
PMID:  18070624     Owner:  NLM     Status:  MEDLINE    
In the present study, the changeover from the Pico.Tag HPLC method to the AccQ.Tag(ultra) UPLC method for the analysis of amino acids in casein and bovine serum albumine hydrolysates is described. The total chromatographic run time of the AccQ.Tag(ultra) UPLC method was only 40% of the time required for the Pico.Tag HPLC method. Quantitative results of both methods for casein and bovine serum albumine hydrolysates compared fairly well. The derivatisation protocol for the formation of AQC derivatives of amino acids was automated using a Gilson Model 215 liquid handler. Comparison of the manual derivatisation protocol with the automated protocol showed lower coefficients of variation for the latter. Combination of the AccQ.Tag(ultra) UPLC method and automated derivatisation resulted in improved throughput compared to the Pico.Tag HPLC method.
Ilco Boogers; Wim Plugge; Yair Q Stokkermans; Alexander L L Duchateau
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Publication Detail:
Type:  Journal Article     Date:  2007-11-22
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1189     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-18     Completed Date:  2008-07-18     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  406-9     Citation Subset:  IM    
DSM Food Specialties, R&D/Analysis Department, P.O. Box 1, 2600 MA Delft, The Netherlands.
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MeSH Terms
Amino Acids / analysis*,  chemistry
Chromatography, High Pressure Liquid / instrumentation,  methods*
Protein Hydrolysates / analysis*,  chemistry
Reproducibility of Results
Reg. No./Substance:
0/Amino Acids; 0/Protein Hydrolysates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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