Document Detail


Ultra Scale-Down device to predict dewatering levels of solids recovered in a continuous scroll decanter centrifuge.
MedLine Citation:
PMID:  24124159     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
During centrifugation operation, the major challenge in the recovery of extracellular proteins is the removal of the maximum liquid entrapped within the spaces between the settled solids - dewatering level. The ability of the scroll decanter centrifuge (SDC) to process continuously large amounts of feed material with high concentration of solids without the need for re-suspension of feeds, and also to achieve relatively high dewatering, could be of great benefit for future use in the biopharmaceutical industry. However, for reliable prediction of dewatering in such a centrifuge, tests using the same kind of equipment at pilot-scale are required, which are time consuming and costly. In order to alleviate the need of pilot-scale trials, a novel USD device, with reduced amounts of feed (2 ml) and to be used in the laboratory, was developed to predict the dewatering levels of a SDC. To verify USD device, dewatering levels achieved were plotted against equivalent compression (Gtcomp ) and decanting (Gtdec ) times, obtained from scroll rates and feed flow rates operated at pilot-scale, respectively. The USD device was able to successfully match dewatering trends of the pilot-scale as a function of both Gtcomp and Gtdec , particularly for high cell density feeds, hence accounting for all key variables that influenced dewatering in a SDC. In addition, it accurately mimicked the maximum dewatering performance of the pilot-scale equipment. Therefore the USD device has the potential to be a useful tool at early stages of process development to gather performance data in the laboratory thus minimising lengthy and costly runs with pilot-scale SDC. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013.
Authors:
A G Lopes; E Keshavarz-Moore
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-1
Journal Detail:
Title:  Biotechnology progress     Volume:  -     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 American Institute of Chemical Engineers (AIChE).
Affiliation:
The Advanced Centre of Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom.
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