Document Detail

Ulinastatin as a neuroprotective and anti-inflammatory agent in infant piglets model undergoing surgery on hypothermic low-flow cardiopulmonary bypass.
MedLine Citation:
PMID:  23384299     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE: Infants are potentially more susceptible to brain injury mediated via cell death attributed to cardiopulmonary bypass (CPB) especially with prolonged hypothermic low flow (HLF). We hypothesized that a human urinary protease inhibitor (ulinastatin), by its anti-inflammatory effect, would reduce central nervous system (CNS) injury during HLF.
METHODS: Fifteen general-type infant piglets were randomized to ulinastatin group (Group U, n = 5), control group (Group C, n = 5), and sham operation group (Group S, n = 5). Routine CPB was established after median thoracotomy in Group U and C under anesthesia. When the temperature of infant piglets dropped down to 25°C, low-flow CPB (50 ml·kg(-1) ·min(-1) ) was instituted. After 120 min of aortic cross-clamping and 20- to 30-min rewarming, the aortic cross-clamp was removed and finally the piglet was weaned from CPB. Five thousand units per killogram of ulinastatin and equivalently normal saline were, respectively, given at the beginning of and at aortic declamping in Group U and Group C. Group S just received sham median thoracotomy. Venous blood samples were taken immediately after anesthesia induction in all three groups, 5- and 120-min post CPB in both Group U and C, respectively; plasma markers of inflammation and CNS injury were compared. Pathology results of hippocampus were observed by light microscopy.
RESULTS: Statistically significant differences between Group C and U were noted in the expression of inflammatory markers such as IL-10, TNF-α and neuron-specific enolase at 120-min post CPB. Brain injuries were observed in both groups (index cases and controls) and were milder in Group U.
CONCLUSIONS: In our study, HLF CPB on infant piglets resulted in brain injury, and ulinastatin might reduce the extent of such injury.
Xiaocou Wang; Qinghua Xue; Fuxia Yan; Lihuan Li; Jinping Liu; Shoujun Li; Shengshou Hu
Related Documents :
23180489 - Decreased spontaneous arousability in preterm newborns with impaired neurological outcome.
25000829 - Risk factors for ventilator-associated pneumonia in neonatal intensive care unit patients.
16131989 - Is the correction factor used in the breath test assessment of gastric emptying appropr...
23221579 - Long-chain pufa supplementation in rural african infants: a randomized controlled trial...
7794709 - Mineral excretion following furosemide compared with bumetanide therapy in premature in...
22627869 - Pharmacokinetics and tolerability of single-dose daptomycin in young infants.
662489 - Flow-volume relationship at low lung volumes in healthy term newborn infants.
19640269 - High frequencies of elevated alkaline phosphatase activity and rickets exist in extreme...
12152519 - Nevirapine resistance after single dose prophylaxis.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Paediatric anaesthesia     Volume:  23     ISSN:  1460-9592     ISO Abbreviation:  Paediatr Anaesth     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206575     Medline TA:  Paediatr Anaesth     Country:  France    
Other Details:
Languages:  eng     Pagination:  209-16     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Department of Anesthesiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environm...
Next Document:  Protective effect of esmolol on myocardial ischemic injury during open heart surgery in children.