Document Detail


Ubiquitin-ribosomal protein S27a gene overexpressed in human colorectal carcinoma is an early growth response gene.
MedLine Citation:
PMID:  8385574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the extension proteins on the carboxy terminus of ubiquitin was reported as the ribosomal protein S27a. We have cloned a gene which encodes this ubiquitin hybrid protein from a complementary DNA library of a human colon carcinoma cell line. Northern blot analysis of surgical specimens from colon cancer patients showed that these messenger RNA levels were higher in tumor tissue than in adjacent normal mucosa. Furthermore, to investigate the role of this novel ubiquitin hybrid gene in cellular growth control, the responsiveness of this gene to serum growth factors was examined. Within 30 min after serum or 12-O-tetradecanoylphorbol-13-acetate stimulation, its messenger RNA expression in rat fibroblast cells (Rat 1) was increased. Nuclear runoff transcription studies showed that the kinetics of induction of this gene is almost identical to that of protooncogene c-jun or c-fos, the known early growth response genes. Thus, this ubiquitin hybrid gene appears to be a novel early growth response gene overexpressed in human colon cancer and warrants further studies in the pathogenesis of colorectal carcinoma.
Authors:
J M Wong; K Mafune; H Yow; E N Rivers; T S Ravikumar; G D Steele; L B Chen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  53     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-05-11     Completed Date:  1993-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1916-20     Citation Subset:  IM    
Affiliation:
Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Colonic Neoplasms / genetics*
Gene Expression*
Humans
Metalloproteins*
Molecular Sequence Data
Nuclear Proteins*
Proto-Oncogenes*
RNA, Messenger / analysis
RNA-Binding Proteins
Rats
Ribosomal Proteins / genetics*
Transcription, Genetic
Tumor Cells, Cultured
Ubiquitins / genetics*
Grant Support
ID/Acronym/Agency:
P01-CA44704-02/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Metalloproteins; 0/Nuclear Proteins; 0/RNA, Messenger; 0/RNA-Binding Proteins; 0/RPS27 protein, human; 0/Ribosomal Proteins; 0/Rps27 protein, rat; 0/Ubiquitins

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