| Ubiquitin metabolism in cycling Xenopus egg extracts. | |
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MedLine Citation:
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PMID: 8407956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xenopus egg extract is capable of supporting mitosis in vitro, which makes it ideal for biochemical analysis of the cell cycle. Since several studies have implicated the ubiquitin system in cell cycle progression, we have measured ubiquitin conjugation rates, proteolysis of ubiquitin-lysozyme conjugates, and rates of isopeptidase activity in cycling Xenopus egg extracts. Although ubiquitin conjugation in cytostatic factor arrested extract was half that in activated extract, there were no changes in rates of ubiquitin conjugation during the cell cycle. Ubiquitin conjugates are degraded by a 26 S ATP-stimulated protease. The ability of the 26 S protease to degrade ubiquitin-lysozyme conjugates and a fluorigenic peptide also remained constant across the cell cycle. In contrast to previously characterized systems, isopeptidase activity in Xenopus egg extract is energy-dependent. Glycerol gradient fractionation of Xenopus egg extract separated two ATP-dependent isopeptidases. On co-sedimented with the 26 S protease; the other sedimented slower and was not associated with any additional proteolytic activity. As found for rates of Ub conjugation and conjugate proteolysis, there was little or no variation in isopeptidase activity during the cell cycle. |
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Authors:
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D Mahaffey; Y Yoo; M Rechsteiner |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 268 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-24 Completed Date: 1993-11-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 21205-11 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon-Nitrogen Lyases* Cell Cycle* Female Hydrolysis Lyases / metabolism Muramidase / metabolism Oocytes / cytology, enzymology, metabolism Ubiquitins / metabolism* Xenopus laevis |
| Grant Support | |
ID/Acronym/Agency:
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5T32 CA09602/CA/NCI NIH HHS; GM 27159/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ubiquitins; EC 3.2.1.17/Muramidase; EC 4.-/Lyases; EC 4.3.-/Carbon-Nitrogen Lyases; EC 4.3.-/isopeptidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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