Document Detail


Ubiquitin E3 Ligase A20 Contributes to Maintaining Epithelial Barrier Function.
MedLine Citation:
PMID:  22854680     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background and Aims: Epithelial barrier dysfunction is involved in the pathogenesis of allergic diseases; the mechanism is to be further understood. Ubiquitin E3 ligase A20 (A20) plays a role in maintaining the homeostasis in the body. This study aims to investigate the role of A20 in maintaining the epithelial barrier function. Methods: Human intestinal epithelial cell line, Caco-2 cells, was cultured to monolayers to test the endocytosis and degradation of a model allergen, ovalbumin (OVA). The role of A20 in the endosome/lysosome fusion in epithelial cells was tested with A20-sufficient and A20-deficient Caco-2 cells and visualized by immunocytochemistry. Results: Caco-2 cells could endocytose exogenous allergens (OVA) in culture. The endocytic OVA was degraded in A20-sufficient Caco-2 cells via the mechanism of endosome/lysosome fusion, while the A20-deficient Caco-2 monolayers converted the OVA to the basal compartment of transwells, which conserved the antigenicity reflected by that it induced T cell proliferation in an allergen-specific manner. A20 was required in the fusion of endosomes and lysosomes. Conclusion: A20 contributes to maintaining the epithelial barrier function.
Authors:
Peixin Huang; Xiao-Rui Geng; Gui Yang; Chi Chen; Zhanju Liu; Ping-Chang Yang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-01
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  30     ISSN:  1421-9778     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  702-710     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Gastroenterology, the Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
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