Document Detail


UbcH10 expression provides a useful tool for the prognosis and treatment of non-small cell lung cancer.
MedLine Citation:
PMID:  22760214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the expression pattern of UbcH10 in non-small cell lung cancer (NSCLC) and its correlations with clinicopathological features and prognostic value in NSCLC patients.
METHODS: The UbcH10 expression in NSCLC tissues, SK-MES-1, and A549 lung cancer cell lines was evaluated, and its correlation with clinicopathological features and prognostic value in NSCLC patients was examined by Kaplan-Meier analysis and Cox regression analysis. The biological effects of UbcH10 on the cell proliferation, cell cycle, and chemosensitivity to gemcitabine or paclitaxel in SK-MES-1 cells were examined upon the UbcH10 silencing.
RESULTS: UbcH10 is overexpressed in poorly differentiated NSCLC samples than in well-differentiated ones, and its expression level was significantly higher in squamous cell carcinoma than that in adenocarcinoma. Higher UbcH10 expression was associated with a shorter postoperative survival time of NSCLC patients by Kaplan-Meier method and was found to be an independent risk factor that influences the postoperative survival time of NSCLC patients by Cox regression analysis. UbcH10 mRNA and protein expression were significantly upregulated in SK-MES-1 cells compared with A549 and MRC-5 cells. Suppression of UbcH10 expression in SK-MES-1 cells inhibited cell proliferation and increased chemosensitivity to gemcitabine or paclitaxel concomitant with decreased MDR1 gene expression.
CONCLUSION: UbcH10 may play an important role in NSCLC carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC.
Authors:
Liming Zhao; Lei Jiang; Liangzhe Wang; Jin He; Hongyu Yu; Guangyuan Sun; Jiquan Chen; Qingyu Xiu; Bing Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-04
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  138     ISSN:  1432-1335     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-26     Completed Date:  2013-01-23     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1951-61     Citation Subset:  IM    
Affiliation:
Department of Respiratory Disease, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China. 1976zlm@163.com
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology
Blotting, Western
Carcinoma, Non-Small-Cell Lung / drug therapy,  genetics,  metabolism*
Cell Cycle / drug effects
Cell Line
Cell Line, Tumor
Cell Proliferation / drug effects
Deoxycytidine / analogs & derivatives,  pharmacology
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry / statistics & numerical data
Kaplan-Meier Estimate
Lung / drug effects,  metabolism*,  pathology
Lung Neoplasms / drug therapy,  genetics,  metabolism*
Male
Middle Aged
Paclitaxel / pharmacology
Prognosis
Proportional Hazards Models
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological / genetics,  metabolism*
Ubiquitin-Conjugating Enzymes / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Tumor Markers, Biological; 33069-62-4/Paclitaxel; 951-77-9/Deoxycytidine; B76N6SBZ8R/gemcitabine; EC 6.3.2.19/UBE2C protein, human; EC 6.3.2.19/Ubiquitin-Conjugating Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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