| The Ubc3 (Cdc34) ubiquitin-conjugating enzyme is ubiquitinated and phosphorylated in vivo. | |
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MedLine Citation:
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PMID: 8164658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transition from G1 to S phase of the cell cycle in Saccharomyces cerevisiae requires the activity of the Ubc3 (Cdc34) ubiquitin-conjugating enzyme. S. cerevisiae cells lacking a functional UBC3 (CDC34) gene are able to execute the Start function that initiates the cell cycle but fail to form a mitotic spindle or enter S phase. The Ubc3 (Cdc34) enzyme has previously been shown to catalyze the attachment of multiple ubiquitin molecules to model substrates, suggesting that the role of this enzyme in cell cycle progression depends on its targeting an endogenous protein(s) for degradation. In this report, we demonstrate that the Ubc3 (Cdc34) protein is itself a substrate for both ubiquitination and phosphorylation. Immunochemical localization of the gene product to the nucleus renders it likely that the relevant substrates similarly reside within the nucleus. |
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Authors:
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M G Goebl; L Goetsch; B Byers |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 14 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-05-25 Completed Date: 1994-05-25 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3022-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Cycle / genetics* Cell Nucleus / enzymology, ultrastructure Fungal Proteins / biosynthesis G1 Phase Genes, Fungal Ligases / analysis, biosynthesis*, genetics Phosphorylation Plasmids Protein Processing, Post-Translational* Restriction Mapping S Phase Saccharomyces cerevisiae / cytology, enzymology*, genetics* Sequence Deletion Ubiquitin-Protein Ligase Complexes* Ubiquitin-Protein Ligases Ubiquitins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AG00057/AG/NIA NIH HHS; GM18541/GM/NIGMS NIH HHS; GM45460/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Ubiquitins; EC 6.-/Ligases; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/anaphase-promoting complex |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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