Document Detail

UVA irradiation-induced activation of activator protein-1 is correlated with induced expression of AP-1 family members in the human keratinocyte cell line HaCaT.
MedLine Citation:
PMID:  11950097     Owner:  NLM     Status:  MEDLINE    
To determine whether the transcription factor activator protein-1 (AP-1) could be modulated by ultraviolet A (UVA) exposure, we examined AP-1 DNA-binding activity and transactivation after exposure to UVA in the human immortalized keratinocyte cell line HaCaT. Maximal AP-1 transactivation was observed with 250 kJ/m2 UVA between 3 and 4 h after irradiation. DNA binding of AP-1 to the target 12-O-tetradecanoylphorbol-13-acetate response element sequence was maximally induced 1-3 h after irradiation. Both de novo transcription and translation contributed to the UVA-induced AP-1 DNA binding. c-Fos was implicated as a primary component of the AP-1 DNA-binding complex. Other components of the complex included Fra-2, c-Jun, JunB and JunD. UVA irradiation induced protein expression of c-Fos, c-Jun, Fra-1 and Fra-2. Phosphorylated forms of these induced proteins were determined at specific time points. A strong correlation existed between UVA-induced AP-1 activity and accumulation of c-Fos, c-Jun and Fra-1 proteins. UVA irradiation also induced c-fos and c-jun mRNA expression and transcriptional activation of the c-fos gene promoter. These results demonstrate that UVA irradiation activates AP-1 and that c-fos induction may play a critical role in the response of these human keratinocytes to UVA irradiation.
Amy L Silvers; G Timothy Bowden
Related Documents :
14512277 - C-jun is regulated by combination of enhanced expression and phosphorylation in acute-o...
9988737 - C-jun is a jnk-independent coactivator of the pu.1 transcription factor.
9757037 - Prostaglandin e2 increases proenkephalin mrna level in rat astrocyte-enriched culture.
12507287 - Expression of c-fos and c-jun protooncogenes in the uteri of immature mice neonatally e...
16306087 - Antagonistic functions of tetradecanoyl phorbol acetate-inducible-sequence 11b and hur ...
24065677 - Construction of recombinant adenoviruses carrying the optimal shrna template against go...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  75     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-12     Completed Date:  2002-05-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  302-10     Citation Subset:  IM    
Department of Radiation Oncology, Arizona Cancer Center, The University of Arizona, Tucson 85724, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Transformed
DNA / metabolism
Keratinocytes / metabolism,  radiation effects*
Transcription Factor AP-1 / metabolism*,  physiology
Transcriptional Activation / physiology
Ultraviolet Rays*
Grant Support
Reg. No./Substance:
0/Transcription Factor AP-1; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Treatment of murine cutaneous melanoma with near infrared light.
Next Document:  Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyr...