Document Detail


UVA irradiation-induced activation of activator protein-1 is correlated with induced expression of AP-1 family members in the human keratinocyte cell line HaCaT.
MedLine Citation:
PMID:  11950097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether the transcription factor activator protein-1 (AP-1) could be modulated by ultraviolet A (UVA) exposure, we examined AP-1 DNA-binding activity and transactivation after exposure to UVA in the human immortalized keratinocyte cell line HaCaT. Maximal AP-1 transactivation was observed with 250 kJ/m2 UVA between 3 and 4 h after irradiation. DNA binding of AP-1 to the target 12-O-tetradecanoylphorbol-13-acetate response element sequence was maximally induced 1-3 h after irradiation. Both de novo transcription and translation contributed to the UVA-induced AP-1 DNA binding. c-Fos was implicated as a primary component of the AP-1 DNA-binding complex. Other components of the complex included Fra-2, c-Jun, JunB and JunD. UVA irradiation induced protein expression of c-Fos, c-Jun, Fra-1 and Fra-2. Phosphorylated forms of these induced proteins were determined at specific time points. A strong correlation existed between UVA-induced AP-1 activity and accumulation of c-Fos, c-Jun and Fra-1 proteins. UVA irradiation also induced c-fos and c-jun mRNA expression and transcriptional activation of the c-fos gene promoter. These results demonstrate that UVA irradiation activates AP-1 and that c-fos induction may play a critical role in the response of these human keratinocytes to UVA irradiation.
Authors:
Amy L Silvers; G Timothy Bowden
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  75     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-12     Completed Date:  2002-05-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  302-10     Citation Subset:  IM    
Affiliation:
Department of Radiation Oncology, Arizona Cancer Center, The University of Arizona, Tucson 85724, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Transformed
DNA / metabolism
Humans
Keratinocytes / metabolism,  radiation effects*
Transcription Factor AP-1 / metabolism*,  physiology
Transcriptional Activation / physiology
Ultraviolet Rays*
Grant Support
ID/Acronym/Agency:
CA23074/CA/NCI NIH HHS; CA27502/CA/NCI NIH HHS; R25CA78447/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Transcription Factor AP-1; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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