Document Detail


UVA-induced cyototoxicity and DNA damaging potential of benz (e) acephenanthrylene.
MedLine Citation:
PMID:  20832460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The toxicity of benz (e) acephenanthrylene (BeA) has been studied earlier with regard to the carcinogenicity of its metabolites, but its phototogenotoxicity is not well understood. Present study aimed to analyze the photodynamic response of BeA in human skin cell line (A375) under ambient environmental intensity of UVA (1.40 mW/cm(2)). Kinetic of BeA showed that the highest intracellular uptake of BeA occurred after 24h of incubation. Cell viability, generation of reactive oxygen species (ROS), oxidative stress and DNA damage induced by BeA under UVA irradiation were assessed. BeA generates singlet oxygen ((1)O(2)), superoxide anion radical (O(2)(ⁱ-)) and hydroxyl radical (ⁱOH) in a concentration-dependent manner. It was observed that glutathione reduced (GSH) and catalase activity were decreased while DNA damage and cell death were induced significantly (P>0.01) as concentration of BeA increased. Thus our results suggest that BeA may be phototoxic as well as photogenotoxic under UVA irradiation.
Authors:
Daoud Ali; R S Ray; R K Hans
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-09
Journal Detail:
Title:  Toxicology letters     Volume:  199     ISSN:  1879-3169     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  193-200     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Photobiology Division, Indian Institute of Toxicology Research (CSIR), Lucknow, Uttar Pradesh, India.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Benz(a)Anthracenes / pharmacology,  toxicity*
Caspase 3 / metabolism
Catalase / analysis
Cell Survival / drug effects
Cells, Cultured
DNA Damage*
Glutathione / analysis
Humans
Reactive Oxygen Species
Ultraviolet Rays / adverse effects*
Chemical
Reg. No./Substance:
0/Benz(a)Anthracenes; 0/Reactive Oxygen Species; 70-18-8/Glutathione; EC 1.11.1.6/Catalase; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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