Document Detail


UV-vis and FT-IR spectra of ultraviolet irradiated collagen in the presence of antioxidant ascorbic acid.
MedLine Citation:
PMID:  20036425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of deleterious UV radiation on collagen molecules in the absence and presence of ascorbic acid using UV-vis and FT-IR spectroscopy has been studied. Intensity of UV-vis absorption spectrum of collagen with a maximum at 275 m due to the aromatic residues (tyrosine and phenylalanine) increases with the increasing dose of UV radiation. This effect is significantly hindered in the presence of antioxidant ascorbic acid. Intensities of FT-IR bands (amide A, B, I and II) of collagen decrease with the increase of the UV radiation dosage. Intensities of bands are also decreased in the presence of ascorbic acid. Results suggest that increasing the concentration of ascorbic acid increases the photo-stability of collagen, and the collagen becomes less sensitive to UV radiation. It is possible that hydrogen bonds form between the groups N-H of collagen and C=O of ascorbic acid. It is believed that under UV radiation free radicals appear in acid soluble collagen and resulting in photodegradation of the macromolecule restore due to the ability of ascorbic acid donating one or two electrons. Increasing the dose of radiation causes more molecules of ascorbic acid to slow down, and their antioxidant effect is diminished accordingly.
Authors:
Nunu O Metreveli; Ketevan K Jariashvili; Louisa O Namicheishvili; David V Svintradze; Eduard N Chikvaidze; Alina Sionkowska; Joanna Skopinska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-29
Journal Detail:
Title:  Ecotoxicology and environmental safety     Volume:  73     ISSN:  1090-2414     ISO Abbreviation:  Ecotoxicol. Environ. Saf.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-04-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805381     Medline TA:  Ecotoxicol Environ Saf     Country:  United States    
Other Details:
Languages:  eng     Pagination:  448-55     Citation Subset:  IM    
Copyright Information:
(c) 2009. Published by Elsevier Inc.
Affiliation:
Faculty of Physics and Mathematics, Ilia Chavchavadze State University, Chavchavadze Ave. 32, 0179 Tbilisi, Georgia.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / chemistry*
Ascorbic Acid / chemistry*
Collagen* / chemistry,  radiation effects
Spectrophotometry, Ultraviolet / methods
Spectroscopy, Fourier Transform Infrared / methods
Ultraviolet Rays / adverse effects*
Chemical
Reg. No./Substance:
0/Antioxidants; 50-81-7/Ascorbic Acid; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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