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UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid.
MedLine Citation:
PMID:  23106169     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We examined the redox effects of UV irradiation on cell wall isolates from Pisum sativum leaves, and polygalacturonic and galacturonic acid, in the presence of hydrogen peroxide. For this purpose, electron paramagnetic resonance spectroscopy and two spin-traps (DEPMPO and BMPO), capable of differentiating between various free radicals, were applied. Systems were exposed to UV-B (maximum emission at 312 nm) and UV-A (352 nm) for 10 minutes (6 J m(-2) s(-1) ). Cell wall isolates exposed to UV in the presence of hydrogen peroxide, produced hydroxyl radical, carbon dioxide radical, and superoxide. The production of superoxide was observed for cell wall isolates, polygalacturonic acid (in the presence and in the absence of calcium) and galacturonic acid, and it was diminished upon superoxide dismutase supplementation. The production is at least partially based on the reaction of hydroxyl radicals with (poly)galacturonic acid having carbon dioxide radicals as a products. Acting as a strong reducing agent, carbon dioxide radical reacts with molecular oxygen to produce superoxide. The results presented here shed a new light on: (i) the redox-modulating role of cell wall; (ii) the production of superoxide in the extracellular compartment; (iii) the mechanisms involved in translating UV stress into molecular signaling; and (iv) some other UV-related phenomena in plants, such as CO(2) emission.
Authors:
Jelena Bogdanović Pristov; Sonja Veljović Jovanović; Aleksandra Mitrović; Ivan Spasojević
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-26
Journal Detail:
Title:  Physiologia plantarum     Volume:  -     ISSN:  1399-3054     ISO Abbreviation:  Physiol Plant     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © Physiologia Plantarum 2012.
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000, Belgrade, Serbia.
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