Document Detail


UV-sensitive photoreceptor protein OPN5 in humans and mice.
MedLine Citation:
PMID:  22043319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A variety of animal species utilize the ultraviolet (UV) component of sunlight as their environmental cues, whereas physiological roles of UV photoreception in mammals, especially in human beings, remain open questions. Here we report that mouse neuropsin (OPN5) encoded by the Opn5 gene exhibited an absorption maximum (λmax) at 380 nm when reconstituted with 11-cis-retinal. Upon UV-light illumination, OPN5 was converted to a blue-absorbing photoproduct (λmax 470 nm), which was stable in the dark and reverted to the UV-absorbing state by the subsequent orange light illumination, indicating its bistable nature. Human OPN5 also had an absorption maximum at 380 nm with spectral properties similar to mouse OPN5, revealing that OPN5 is the first and hitherto unknown human opsin with peak sensitivity in the UV region. OPN5 was capable of activating heterotrimeric G protein Gi in a UV-dependent manner. Immuno-blotting analyses of mouse tissue extracts identified the retina, the brain and, unexpectedly, the outer ears as the major sites of OPN5 expression. In the tissue sections of mice, OPN5 immuno-reactivities were detected in a subset of non-rod/non-cone retinal neurons as well as in the epidermal and muscle cells of the outer ears. Most of these OPN5-immuno-reactivities in mice were co-localized with positive signals for the alpha-subunit of Gi. These results demonstrate the first example of UV photoreceptor in human beings and strongly suggest that OPN5 triggers a UV-sensitive Gi-mediated signaling pathway in the mammalian tissues.
Authors:
Daisuke Kojima; Suguru Mori; Masaki Torii; Akimori Wada; Rika Morishita; Yoshitaka Fukada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-17
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-01     Completed Date:  2012-04-06     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e26388     Citation Subset:  IM    
Affiliation:
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo-Ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
Humans
Membrane Proteins
Mice
Opsins / physiology*
Photic Stimulation
Photoreceptor Cells*
Retinaldehyde
Tissue Distribution
Ultraviolet Rays*
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/OPN5 protein, human; 0/OPN5 protein, mouse; 0/Opsins; 116-31-4/Retinaldehyde; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gi-Go
Comments/Corrections

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