| UV-modulated substrate rigidity for multiscale study of mechanoresponsive cellular behaviors. | |
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MedLine Citation:
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PMID: 22742430 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure baking time, we achieved a broad range of bulk Young's modulus for photoPDMS from 0.027 to 2.48 MPa. Biocompatibility of photoPDMS was assayed, and no significant cytotoxic effect was detected as compared to conventional PDMS. We demonstrated that the bulk Young's modulus of photoPDMS could impact cell morphology, adhesion formation, cytoskeletal structure, and cell proliferation. We further fabricated photoPDMS micropost arrays for multiscale study of mechanoresponsive cellular behaviors. Our results suggested that adherent cells could sense and respond to changes of substrate rigidity at a subfocal adhesion resolution. Together, we demonstrated the potential of photoPDMS as a photosensitive and rigidity-tunable material for mechanobiology studies. |
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Authors:
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Yubing Sun; Liang-Ting Jiang; Ryoji Okada; Jianping Fu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-07-12 |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 28 ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-07-25 Completed Date: 2012-11-30 Revised Date: 2013-03-05 |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 10789-96 Citation Subset: IM |
Affiliation:
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Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / chemistry* Cell Adhesion Cell Proliferation Cells, Cultured Cytoskeleton / chemistry Dimethylpolysiloxanes / chemistry* Fibroblasts / cytology* Mice Molecular Structure NIH 3T3 Cells Photosensitizing Agents / chemistry* Ultraviolet Rays* |
| Grant Support | |
ID/Acronym/Agency:
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UL1 RR024986/RR/NCRR NIH HHS; UL1RR024986/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Dimethylpolysiloxanes; 0/Photosensitizing Agents; 63148-62-9/baysilon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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