Document Detail

UV-modulated substrate rigidity for multiscale study of mechanoresponsive cellular behaviors.
MedLine Citation:
PMID:  22742430     Owner:  NLM     Status:  MEDLINE    
Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure baking time, we achieved a broad range of bulk Young's modulus for photoPDMS from 0.027 to 2.48 MPa. Biocompatibility of photoPDMS was assayed, and no significant cytotoxic effect was detected as compared to conventional PDMS. We demonstrated that the bulk Young's modulus of photoPDMS could impact cell morphology, adhesion formation, cytoskeletal structure, and cell proliferation. We further fabricated photoPDMS micropost arrays for multiscale study of mechanoresponsive cellular behaviors. Our results suggested that adherent cells could sense and respond to changes of substrate rigidity at a subfocal adhesion resolution. Together, we demonstrated the potential of photoPDMS as a photosensitive and rigidity-tunable material for mechanobiology studies.
Yubing Sun; Liang-Ting Jiang; Ryoji Okada; Jianping Fu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-12
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  28     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-25     Completed Date:  2012-11-30     Revised Date:  2013-03-05    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10789-96     Citation Subset:  IM    
Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, United States.
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MeSH Terms
Biocompatible Materials / chemistry*
Cell Adhesion
Cell Proliferation
Cells, Cultured
Cytoskeleton / chemistry
Dimethylpolysiloxanes / chemistry*
Fibroblasts / cytology*
Molecular Structure
NIH 3T3 Cells
Photosensitizing Agents / chemistry*
Ultraviolet Rays*
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Dimethylpolysiloxanes; 0/Photosensitizing Agents; 63148-62-9/baysilon

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