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UV-C-irradiation sublethal stress does not alter antibiotic susceptibility of the viridans group streptococci to β-lactam, macrolide, and fluoroquinolone antibiotic agents.
MedLine Citation:
PMID:  22887906     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Aim:  Previous work has indicated that environmental stresses on bacteria might lead to an upregulation of stress response. LED curing lights (315-400 nm) and other UV lights used in tooth whitening cosmetic procedures might act as stresses. We examined the effect of UV-C light, as a high-energy surrogate to the lower-energy UV-A light used in such instruments, to examine its effect on the antibiotic susceptibility of viridans group streptococci. Methods:  Twelve species of viridans group streptococci were examined in this study: Streptococcus anginosus, Streptococcus australis, Streptococcus cristatus, Streptococcus gordonii, Streptococcus infantis, Streptococcus mitis, Streptococcus mutans, Streptococcus oralis, Streptococcus parasanguinis, Streptococcus pneumoniae, Streptococcus salivarius, and Streptococcus sanguinis. These organisms were exposed to varying degrees of sublethal UV-C radiation, and their minimum inhibitory concentration susceptibility was determined by broth dilution assay against three classes of commonly-used antibiotics: β-lactams (penicillin), macrolides (erythromycin), and fluoroquinolones (ciprofloxacin). Results:  There was no significant difference between antibiotic susceptibility before UV-C exposure and following maximum sublethal stress, prior to cell death due to fatal UV-C exposure. Conclusions:  Exposure to UV-C light will not result in altered antibiotic susceptibility patterns on viridans group streptococci. Given that UV-C is more toxic and mutagenic than UV-A light, it is unlikely than UV-A light would yield any difference in response to such exposure.
Authors:
Yasunori Maeda; Wilson A Coulter; Colin E Goldsmith; B Cherie Millar; John E Moore
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Publication Detail:
Type:  Journal Article     Date:  2011-09-15
Journal Detail:
Title:  Journal of investigative and clinical dentistry     Volume:  3     ISSN:  2041-1626     ISO Abbreviation:  J Investig Clin Dent     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524471     Medline TA:  J Investig Clin Dent     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  198-202     Citation Subset:  D; IM    
Copyright Information:
© 2011 Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland, UK  School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland, UK  School of Dentistry, Queen's University of Belfast, Royal Group of Hospitals, Belfast, Northern Ireland, UK.
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