| USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers. | |
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MedLine Citation:
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PMID: 20549504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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USP32, on chromosomal band 17q23.1-17q23.2, is a highly conserved but uncharacterized gene that gave rise during evolution to a well-known hominoid-specific proto-oncogene, USP6. We investigated the expression profile of USP32 in human tissues and examined its functions to gain insight into this novel member of the well-conserved ubiquitination system. We detected ubiquitous USP32 expression across tissues and confirmed the predicted deubiquitination function owing to the presence of conserved peptidase signature aspargine, cysteine, histidine, and aspartic acid domains of ubiquitin-specific proteases. A Golgi localization of GFP-fused USP32 was detected by fluorescent protection assay and BODIPY-TR staining. In addition, stable silencing of USP32 caused a significant decrease in the proliferation and migration rate of cells. Based on these and the fact that USP32 maps to 17q23, which is commonly amplified in breast cancers, we analyzed USP32 expression in breast cancer cells. We detected high expression of USP32 in 50% (9 of 18) of breast cancer cell lines and 22% (9 of 41) of primary breast tumors compared to mammary epithelial cells. In summary, we report the preliminary characterization of this novel deubiquitinating enzyme on 17q23 and demonstrate its functional role in the ubiquitin system and its potential involvement in tumorigenesis. |
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Authors:
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Shiva Akhavantabasi; Hesna B Akman; Aysegul Sapmaz; Jennifer Keller; Elizabeth M Petty; Ayse E Erson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-13 |
Journal Detail:
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Title: Mammalian genome : official journal of the International Mammalian Genome Society Volume: 21 ISSN: 1432-1777 ISO Abbreviation: Mamm. Genome Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-19 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100916 Medline TA: Mamm Genome Country: United States |
Other Details:
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Languages: eng Pagination: 388-97 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Inonu Bulvari, M.E.T.U, Ankara, Turkey. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Breast Neoplasms / genetics*, metabolism Carcinoma / genetics*, metabolism Cell Line, Tumor Cell Membrane / metabolism Endopeptidases / genetics*, metabolism*, physiology Enzyme Activation / physiology Female Gene Expression Regulation, Neoplastic Hela Cells Humans Molecular Sequence Data Sequence Homology, Amino Acid Ubiquitin Thiolesterase / genetics*, metabolism*, physiology Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA72877-01A1/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.1.2.15/USP10 protein, human; EC 3.1.2.15/Ubiquitin Thiolesterase; EC 3.4.-/Endopeptidases; EC 3.4.99.-/ubiquitin-specific protease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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