Document Detail

UNC-85, a C. elegans homolog of the histone chaperone Asf1, functions in post-embryonic neuroblast replication.
MedLine Citation:
PMID:  18490010     Owner:  NLM     Status:  MEDLINE    
Normal animal development requires accurate cell divisions, not only in the early stages of rapid embryonic cleavages, but also in later developmental stages. The Caenorhabditis elegans unc-85 gene is implicated only in cell divisions that occur post-embryonically, primarily in terminal neuronal lineages. Variable post-embryonic cell division failures in ventral cord motoneuron precursors result in uncoordinated locomotion of unc-85 mutant larvae by the second larval stage. These neuroblast cell division failures often result in unequally sized daughter nuclei, and sometimes in nuclear fusions. Using a combination of conventional mapping techniques and microarray analysis, we cloned the unc-85 gene, and find that it encodes one of two C. elegans homologs of the yeast Anti-silencing function 1 (Asf1) histone chaperone. The unc-85 gene is expressed in replicating cells throughout development, and the protein is localized in nuclei. Examination of null mutants confirms that embryonic neuroblast cell divisions occur normally, but post-embryonic neuroblast cell divisions fail. Analysis of the DNA content of the mutant neurons indicates that defective replication in post-embryonic neuroblasts gives rise to ventral cord neurons with an average DNA content of approximately 2.5 n. We conclude that UNC-85 functions in post-embryonic DNA replication in ventral cord motor neuron precursors.
Iwen F Grigsby; Fern P Finger
Publication Detail:
Type:  Journal Article     Date:  2008-04-22
Journal Detail:
Title:  Developmental biology     Volume:  319     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-16     Completed Date:  2008-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  100-9     Citation Subset:  IM    
Biology Department and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Biotech-BCHM-2, Troy, NY 12180, USA.
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MeSH Terms
Amino Acid Sequence
Caenorhabditis elegans / embryology,  growth & development*
Caenorhabditis elegans Proteins / genetics*,  metabolism*
Cell Nucleus / metabolism
Cloning, Molecular
DNA Replication
Embryo, Nonmammalian / metabolism
Gene Expression Regulation, Developmental
Molecular Chaperones / genetics*,  metabolism*
Molecular Sequence Data
Motor Neurons / metabolism
Neuroepithelial Cells
Sequence Alignment
Reg. No./Substance:
0/ASF1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/Molecular Chaperones

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