Document Detail


UNC-31 (CAPS) is required for dense-core vesicle but not synaptic vesicle exocytosis in Caenorhabditis elegans.
MedLine Citation:
PMID:  17553987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies indicated that CAPS (calcium-dependent activator protein for secretion) functions as an essential component for the Ca2+-dependent exocytosis of dense-core vesicles in neuroendocrine cells. However, recent mouse knock-out studies suggested an alternative role in the vesicular uptake or storage of catecholamines. To genetically assess the functional role of CAPS, we characterized the sole Caenorhabditis elegans CAPS ortholog UNC-31 (uncoordinated family member) and determined its role in dense-core vesicle-mediated peptide secretion and in synaptic vesicle recycling. Novel assays for dense-core vesicle exocytosis were developed by expressing a prepro-atrial natriuretic factor-green fluorescent protein fusion protein in C. elegans. unc-31 mutants exhibited reduced peptide release in vivo and lacked evoked peptide release in cultured neurons. In contrast, cultured neurons from unc-31 mutants exhibited normal stimulated synaptic vesicle recycling measured by FM4-64 [N-(3-triethylammoniumpropyl)-4-(6-(4-diethylamino)phenyl)hexatrienyl)pyridinium dibromide] dye uptake. Conversely, UNC-13, which exhibits sequence homology to CAPS/UNC-31, was found to be essential for synaptic vesicle but not dense-core vesicle exocytosis. These findings indicate that CAPS/UNC-31 function is not restricted to catecholaminergic vesicles but is generally required for and specific to dense-core vesicle exocytosis. Our results suggest that CAPS/UNC-31 and UNC-13 serve parallel and dedicated roles in dense-core vesicle and synaptic vesicle exocytosis, respectively, in the C. elegans nervous system.
Authors:
Sean Speese; Matt Petrie; Kim Schuske; Michael Ailion; Kyoungsook Ann; Kouichi Iwasaki; Erik M Jorgensen; Thomas F J Martin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  27     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-07     Completed Date:  2007-07-03     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6150-62     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Caenorhabditis elegans / genetics,  metabolism*
Caenorhabditis elegans Proteins / biosynthesis,  genetics,  physiology*
Calcium-Binding Proteins / biosynthesis,  genetics,  physiology*
Exocytosis / physiology*
Molecular Sequence Data
Secretory Vesicles / genetics,  metabolism*
Synaptic Vesicles / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
DK40428/DK/NIDDK NIH HHS; MH60997/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Calcium-Binding Proteins; 0/UNC-31 protein, C elegans; 0/Unc-13 protein, C elegans

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