| UNA (unlocked nucleic acid): a flexible RNA mimic that allows engineering of nucleic acid duplex stability. | |
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MedLine Citation:
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PMID: 19604699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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UNA (unlocked nucleic acid) monomers are acyclic derivatives of RNA lacking the C2'-C3'-bond of the ribose ring of RNA. Synthesis of phosphoramidite UNA building blocks of the nucleobases adenine, cytosine, guanine, and uracil is described herein together with their incorporation into RNA strands. UNA monomers additively decrease nucleic acid duplex stability and can be positioned strategically to induce either lack of discrimination of mismatches, that is, universal base behavior, or increased discrimination of mismatches, that is, improved hybridization specificity. UNA-modified RNA duplexes are shown to structurally mimic unmodified RNA duplexes by CD spectroscopy. |
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Authors:
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Niels Langkjaer; Anna Pasternak; Jesper Wengel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-27 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 17 ISSN: 1464-3391 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-21 Completed Date: 2009-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 5420-5 Citation Subset: IM |
Affiliation:
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Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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chemical synthesis,
chemistry* Circular Dichroism Cytosine / chemical synthesis, chemistry* Guanine / chemical synthesis, chemistry* Nucleic Acid Conformation Nucleic Acid Denaturation Organophosphorus Compounds / chemical synthesis, chemistry* RNA / chemical synthesis, chemistry* RNA Stability Temperature Uracil / chemical synthesis, chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Organophosphorus Compounds; 0/phosphoramidite; 63231-63-0/RNA; 66-22-8/Uracil; 71-30-7/Cytosine; 73-24-5/Adenine; 73-40-5/Guanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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