Document Detail

UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.
MedLine Citation:
PMID:  22569263     Owner:  NLM     Status:  In-Data-Review    
Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical. For the first time, the two glucosyltransferases UGT75L6 and UGT94E5 that sequentially mediate the final glucosylation steps in crocin biosynthesis in G. jasminoides have been identified and functionally characterized. UGT75L6 preferentially glucosylates the carboxyl group of crocetin yielding crocetin glucosyl esters, while UGT94E5 glucosylates the 6' hydroxyl group of the glucose moiety of crocetin glucosyl esters. The expression pattern of neither UGT75L6 nor UGT94E5 correlated with the pattern of crocin accumulation in G. jasminoides.
Mai Nagatoshi; Kazuyoshi Terasaka; Miki Owaki; Makiko Sota; Tatsunori Inukai; Akito Nagatsu; Hajime Mizukami
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Publication Detail:
Type:  Journal Article     Date:  2012-03-10
Journal Detail:
Title:  FEBS letters     Volume:  586     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-05-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1055-61     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
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