Document Detail


The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids.
MedLine Citation:
PMID:  16214889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important regulatory role in the activity of steroids in mammals and insects. This study provides an indication that a UGT accepting plant steroids as substrates functions in brassinosteroid (BR) homeostasis. The UGT73C5 of Arabidopsis thaliana catalyses 23-O-glucosylation of the BRs brassinolide (BL) and castasterone. Transgenic plants overexpressing UGT73C5 displayed BR-deficient phenotypes and contained reduced amounts of BRs. The phenotype, which was already apparent in seedlings, could be rescued by application of BR. In feeding experiments with BL, wild-type seedlings converted BL to the 23-O-glucoside; in the transgenic lines silenced in UGT73C5 expression, no 23-O-glucoside was detected, implying that this UGT is the only enzyme that catalyzes BL-23-O-glucosylation in seedlings. Plant lines in which UGT73C5 expression was altered also displayed hypocotyl phenotypes previously described for seedlings in which BR inactivation by hydroxylation was changed. These data support the hypothesis that 23-O-glucosylation of BL is a function of UGT73C5 in planta, and that glucosylation regulates BR activity.
Authors:
Brigitte Poppenberger; Shozo Fujioka; Kazuo Soeno; Gilu L George; Fabián E Vaistij; Sayoko Hiranuma; Hideharu Seto; Suguru Takatsuto; Gerhard Adam; Shigeo Yoshida; Dianna Bowles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  102     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-20     Completed Date:  2005-12-05     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15253-8     Citation Subset:  IM    
Affiliation:
Center for Novel Agricultural Products, Department of Biology, University of York, YO10 5DD, York, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / enzymology*
Arabidopsis Proteins / genetics,  metabolism*
Brassinosteroids
Cholestanols / metabolism*
Glucosyltransferases / genetics,  metabolism*
Phenotype
Plant Growth Regulators / metabolism
Promoter Regions, Genetic
RNA Interference
Steroids, Heterocyclic / metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Brassinosteroids; 0/Cholestanols; 0/Plant Growth Regulators; 0/Steroids, Heterocyclic; 80736-41-0/castasterone; EC 2.4.1.-/Dogt1 protein, Arabidopsis; EC 2.4.1.-/Glucosyltransferases; Y9IQ1L53OX/brassinolide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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