Document Detail

A UFLC-MS/MS method for simultaneous quantitation of spinosin, mangiferin and ferulic acid in rat plasma: application to a comparative pharmacokinetic study in normal and insomnic rats.
MedLine Citation:
PMID:  23019165     Owner:  NLM     Status:  MEDLINE    
Suan-Zao-Ren (SZR) decoction, consisting of Ziziphi Spinosae Semen, Poria, Chuanxiong Rhizoma, Anemarrhenae Rhizoma and Glycyrrhizae Radix Et Rhizoma, is a Traditional Chinese Medicine prescription, clinically used for the treatment of insomnia. The objective of this study was to develop a sensitive and reliable UFLC-MS/MS method for simultaneous quantitation of spinosin, mangiferin and ferulic acid, the main active ingredients in SZR decoction, and to compare the pharmacokinetics of these active ingredients in normal and insomnic rats orally administrated with the prescription. Analytes and IS were separated on a Shim-pack XR-ODS column (75 mm × 3.0 mm, 2.2 µm particles) using gradient elution with the mobile phase consisting of methanol and 0.1% formic acid in water at a flow rate of 0.4 mL/min. The detection of the analytes was performed on 4000Q UFLC-MS/MS system with turbo ion spray source in the negative ion and multiple reaction-monitoring mode. The lower limits of quantification were 1, 6 and 1 ng/mL for spinosin, mangiferin and ferulic acid, respectively. Intra- and inter-day precision and accuracy of analytes were well within acceptance criteria (15%). The mean extraction recoveries of analytes and IS from rats plasma were all more than 85.0%. The validated method has been successfully applied to comparing pharmacokinetic profiles of analytes in rat plasma. The results indicated that no significant difference in pharmacokinetic parameters of ferulic acid was observed between two groups, while absorptions of spinosin and mangiferin in insomnic group were significantly lower than those in normal group.
Bosai He; Qing Li; Ying Jia; Longshan Zhao; Feng Xiao; Chunxiao Lv; Huarong Xu; Xiaohui Chen; Kaishun Bi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  47     ISSN:  1096-9888     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-01-23     Revised Date:  2013-01-28    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1333-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
School of Pharmacy and Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, PR China.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Coumaric Acids / blood*,  pharmacokinetics
Drug Stability
Drugs, Chinese Herbal / administration & dosage,  pharmacokinetics
Flavonoids / blood*,  pharmacokinetics
Linear Models
Rats, Sprague-Dawley
Reproducibility of Results
Sleep Initiation and Maintenance Disorders / blood,  metabolism*
Tandem Mass Spectrometry / methods*
Xanthones / blood*,  pharmacokinetics
Reg. No./Substance:
0/Coumaric Acids; 0/Drugs, Chinese Herbal; 0/Flavonoids; 0/Xanthones; 0/suanzaoren; 1M84LD0UMD/mangiferin; 72063-39-9/spinosin; AVM951ZWST/ferulic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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