Document Detail


A UDP-glucose derivative is required for vacuolar autophagic cell death.
MedLine Citation:
PMID:  18424909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autophagic cell death in Dictyostelium can be dissociated into a starvation-induced sensitization stage and a death induction stage. A UDP-glucose pyrophosphorylase (ugpB) mutant and a glycogen synthase (glcS) mutant shared the same abnormal phenotype. In vitro, upon starvation alone mutant cells showed altered contorted morphology, indicating that the mutations affected the pre-death sensitization stage. Upon induction of cell death, most of these mutant cells underwent death without vacuolization, distinct from either autophagic or necrotic cell death. Autophagy itself was not grossly altered as shown by conventional and electron microscopy. Exogenous glycogen or maltose could complement both ugpB(-) and glcS(-) mutations, leading back to autophagic cell death. The glcS(-) mutation could also be complemented by 2-deoxyglucose that cannot undergo glycolysis. In agreement with the in vitro data, upon development glcS(-) stalk cells died but most were not vacuolated. We conclude that a UDP-glucose derivative (such as glycogen or maltose) plays an essential energy-independent role in autophagic cell death.
Authors:
Emilie Tresse; Artemis Kosta; Corinne Giusti; Marie-Françoise Luciani; Pierre Golstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-10
Journal Detail:
Title:  Autophagy     Volume:  4     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-08-19     Revised Date:  2011-06-30    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  680-91     Citation Subset:  IM    
Affiliation:
Centre d'Immunologie de Marseille-Luminy (CIML), Faculté des Sciences de Luminy, Aix Marseille Université, INSERM U631, CNRS UMR6102, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy / genetics,  physiology*
Dictyostelium / cytology,  enzymology,  genetics
Glycogen Synthase / genetics,  physiology
Mutagenesis, Insertional
UTP-Glucose-1-Phosphate Uridylyltransferase / genetics
Uridine Diphosphate Glucose / analogs & derivatives*,  physiology*
Vacuoles / enzymology,  genetics,  pathology*
Chemical
Reg. No./Substance:
133-89-1/Uridine Diphosphate Glucose; EC 2.4.1.11/Glycogen Synthase; EC 2.7.7.9/UTP-Glucose-1-Phosphate Uridylyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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