Document Detail

UCI-VULV-1, a vulvar squamous carcinoma cell line.
MedLine Citation:
PMID:  7729733     Owner:  NLM     Status:  MEDLINE    
Squamous carcinoma of the vulva (SCV) is an uncommon neoplasm of uncertain etiology. There is evidence that there are two subgroups of SCV, one associated with human papilloma virus (HPV) and a second HPV-negative group. The UCI-VULV-1 cell line, obtained from a lymph node metastasis of an SCV, grows with a population doubling time of approximately 60 hr. The saturation density is 10(5) cells/cm2. The cell line does not exhibit anchorage independence and is weakly tumorigenic. The cells range in appearance from an abundant spindle cell to a less common larger, flat cell. All of the cells are immunoreactive for high-molecular-weight keratin, but only the flat cells, which form squamous pearls in vivo, are immunoreactive for low-molecular-weight keratin. The cell line expresses epidermal growth factor (EGF), transforming growth factor-alpha, the EGF receptor, and p53 protein. Polymerase chain reaction revealed no HPV DNA within the cells. Early passage cells exhibited karyotypic heterogeneity with few similarities to previous described SCV karyotypes. The cells display sensitivity to cis-platinum in concentrations toxic to many ovarian and cervical carcinoma lines. UCI-VULV-1 may be helpful for studying the properties of the HPV-negative form of SCV.
P M Carpenter; G Gamboa-Vujicic; J T Mascarello; S Wilczynski; M Bhaumik; G Dorion; A Manetta
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gynecologic oncology     Volume:  57     ISSN:  0090-8258     ISO Abbreviation:  Gynecol. Oncol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-05-30     Completed Date:  1995-05-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365304     Medline TA:  Gynecol Oncol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
Department of Pathology University of California, Irvine 92668, USA.
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MeSH Terms
Carcinoma, Squamous Cell / pathology*
Cell Division
DNA Probes, HPV
Tumor Cells, Cultured* / pathology,  virology
Vulvar Neoplasms / pathology*
Grant Support
Reg. No./Substance:
0/DNA Probes, HPV

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