Document Detail


U373-MG response to interleukin-1beta-induced oxidative stress.
MedLine Citation:
PMID:  11032359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidative stress has been involved in various neurological disorders and, in the central nervous system, astrocytes represent the cell type that contributes to neuroprotection via glutathione (GSH) metabolism, GSH-metabolizing enzymes like gamma-glutamyltransferase (GGT), and apoE secretion. In this study, using IL-1beta, a proinflammatory and prooxidant cytokine that is increased in numerous pathological situations, cells of astrocytoma cell line U373-MG were exposed to an oxidative stress, leading to c-Jun and c-Fos activation. IL-1beta decreased both GGT activity and intracellular GSH content and increased apoE secretion, initiating astroglial response to injury. We observed that antioxidants inhibit IL-1beta effects on c-Jun and c-Fos proteins, GGT activity and the GSH pool but not on apoE secretion. Our results allow us to conclude that neurological disorders associated with an IL-1beta-induced oxidative stress could be, at least experimentally, reversible in the presence of one antioxidant, N-acetylcysteine.
Authors:
C Malaplate-Armand; Y Gueguen; P Bertrand; L Ferrari; A M Batt
Related Documents :
24393059 - Anti-inflammatory effect of euphorbia hirta in an adjuvant-induced arthritic murine model.
23225169 - Smac mimetic birinapant induces apoptosis and enhances trail potency in inflammatory br...
23545299 - Hookworm excretory/secretory products induce interleukin-4 (il-4)+ il-10+ cd4+ t cell r...
24683439 - Effects of downregulation of microrna-181a on h 2 o 2 -induced h9c2 cell apoptosis via ...
18365879 - Paraquat induces apoptosis in human lymphocytes: protective and rescue effects of gluco...
24372399 - The bacterial effector hopm1 suppresses pamp-triggered oxidative burst and stomatal imm...
21083209 - Unwanted immunogenicity: lessons learned and future challenges.
15537749 - Aluminum-maltolate induces apoptosis and necrosis in neuro-2a cells: potential role for...
21107279 - Levosimendan modulates programmed forms of cell death through k(atp) channels and nitri...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology and toxicology     Volume:  16     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-19     Completed Date:  2001-02-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  155-63     Citation Subset:  IM    
Affiliation:
Centre du Médicament, UPRES 3117, Faculté de Pharmacie, Université Henri Poincaré, Nancy, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Apolipoproteins E / metabolism,  secretion
Ascorbic Acid / pharmacology
Astrocytoma*
Blotting, Western
Cell Survival
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Free Radical Scavengers / pharmacology
Glutathione / metabolism
Humans
Interleukin-1 / pharmacology*
Oxidative Stress / drug effects,  physiology*
Proto-Oncogene Proteins c-fos / analysis
Proto-Oncogene Proteins c-jun / analysis
Tumor Cells, Cultured / drug effects,  enzymology
Vitamin E / pharmacology
gamma-Glutamyltransferase / metabolism
Chemical
Reg. No./Substance:
0/Apolipoproteins E; 0/Free Radical Scavengers; 0/Interleukin-1; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Potential use of isolated glomeruli and cultured mesangial cells as in vitro models to assess nephro...
Next Document:  Dehydroepiandrosterone and melatonin prevent Bacillus anthracis lethal toxin-induced TNF production ...