Document Detail

U373-MG response to interleukin-1beta-induced oxidative stress.
MedLine Citation:
PMID:  11032359     Owner:  NLM     Status:  MEDLINE    
Oxidative stress has been involved in various neurological disorders and, in the central nervous system, astrocytes represent the cell type that contributes to neuroprotection via glutathione (GSH) metabolism, GSH-metabolizing enzymes like gamma-glutamyltransferase (GGT), and apoE secretion. In this study, using IL-1beta, a proinflammatory and prooxidant cytokine that is increased in numerous pathological situations, cells of astrocytoma cell line U373-MG were exposed to an oxidative stress, leading to c-Jun and c-Fos activation. IL-1beta decreased both GGT activity and intracellular GSH content and increased apoE secretion, initiating astroglial response to injury. We observed that antioxidants inhibit IL-1beta effects on c-Jun and c-Fos proteins, GGT activity and the GSH pool but not on apoE secretion. Our results allow us to conclude that neurological disorders associated with an IL-1beta-induced oxidative stress could be, at least experimentally, reversible in the presence of one antioxidant, N-acetylcysteine.
C Malaplate-Armand; Y Gueguen; P Bertrand; L Ferrari; A M Batt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology and toxicology     Volume:  16     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-19     Completed Date:  2001-02-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  155-63     Citation Subset:  IM    
Centre du Médicament, UPRES 3117, Faculté de Pharmacie, Université Henri Poincaré, Nancy, France.
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MeSH Terms
Acetylcysteine / pharmacology
Apolipoproteins E / metabolism,  secretion
Ascorbic Acid / pharmacology
Blotting, Western
Cell Survival
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Free Radical Scavengers / pharmacology
Glutathione / metabolism
Interleukin-1 / pharmacology*
Oxidative Stress / drug effects,  physiology*
Proto-Oncogene Proteins c-fos / analysis
Proto-Oncogene Proteins c-jun / analysis
Tumor Cells, Cultured / drug effects,  enzymology
Vitamin E / pharmacology
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
0/Apolipoproteins E; 0/Free Radical Scavengers; 0/Interleukin-1; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; EC

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