| U373-MG response to interleukin-1beta-induced oxidative stress. | |
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MedLine Citation:
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PMID: 11032359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative stress has been involved in various neurological disorders and, in the central nervous system, astrocytes represent the cell type that contributes to neuroprotection via glutathione (GSH) metabolism, GSH-metabolizing enzymes like gamma-glutamyltransferase (GGT), and apoE secretion. In this study, using IL-1beta, a proinflammatory and prooxidant cytokine that is increased in numerous pathological situations, cells of astrocytoma cell line U373-MG were exposed to an oxidative stress, leading to c-Jun and c-Fos activation. IL-1beta decreased both GGT activity and intracellular GSH content and increased apoE secretion, initiating astroglial response to injury. We observed that antioxidants inhibit IL-1beta effects on c-Jun and c-Fos proteins, GGT activity and the GSH pool but not on apoE secretion. Our results allow us to conclude that neurological disorders associated with an IL-1beta-induced oxidative stress could be, at least experimentally, reversible in the presence of one antioxidant, N-acetylcysteine. |
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Authors:
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C Malaplate-Armand; Y Gueguen; P Bertrand; L Ferrari; A M Batt |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell biology and toxicology Volume: 16 ISSN: 0742-2091 ISO Abbreviation: Cell Biol. Toxicol. Publication Date: 2000 |
Date Detail:
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Created Date: 2001-01-19 Completed Date: 2001-02-15 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8506639 Medline TA: Cell Biol Toxicol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 155-63 Citation Subset: IM |
Affiliation:
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Centre du Médicament, UPRES 3117, Faculté de Pharmacie, Université Henri Poincaré, Nancy, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Apolipoproteins E / metabolism, secretion Ascorbic Acid / pharmacology Astrocytoma* Blotting, Western Cell Survival Dose-Response Relationship, Drug Enzyme Activation / drug effects Free Radical Scavengers / pharmacology Glutathione / metabolism Humans Interleukin-1 / pharmacology* Oxidative Stress / drug effects, physiology* Proto-Oncogene Proteins c-fos / analysis Proto-Oncogene Proteins c-jun / analysis Tumor Cells, Cultured / drug effects, enzymology Vitamin E / pharmacology gamma-Glutamyltransferase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Free Radical Scavengers; 0/Interleukin-1; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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