| Tyrosine sulphation is not required for microvillar expression of intestinal aminopeptidase N. | |
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MedLine Citation:
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PMID: 2902847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 2,6-dichloro-4-nitrophenol (DCNP), an inhibitor of phenol sulphotransferases (EC 2.8.2.-), on the biosynthesis of aminopeptidase N (EC 3.4.11.2) was studied in organ-cultured pig intestinal mucosal explants. At 50 microM DCNP did not affect protein synthesis but it decreased incorporation of [35S]sulphate into aminopeptidase N and other major microvillar hydrolases by 70-85% compared with controls, indicating an inhibition of their post-translational tyrosine sulphation. In labelling experiments with [35S]methionine from 0.5 to 5 h, DCNP was tested for its possible influence on synthesis, processing and microvillar expression of aminopeptidase N, but no effect on any of these parameters could be detected. It can therefore be concluded that tyrosine sulphation is not required (for instance as a sorting signal) for the targeting of newly synthesized enzymes to the microvillar membrane. |
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Authors:
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E M Danielsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 254 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-11-22 Completed Date: 1988-11-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 219-22 Citation Subset: IM |
Affiliation:
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Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminopeptidases
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biosynthesis* Animals Antigens, CD13 Electrophoresis, Polyacrylamide Gel Enzyme Induction / drug effects Intestinal Mucosa / drug effects, enzymology* Microvilli / drug effects, enzymology Nitrophenols / pharmacology Organ Culture Techniques Protein Biosynthesis Sulfur Radioisotopes Swine Tyrosine / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Nitrophenols; 0/Sulfur Radioisotopes; 55520-40-6/Tyrosine; 618-80-4/2,6-dichloro-4-nitrophenol; EC 3.4.11.-/Aminopeptidases; EC 3.4.11.2/Antigens, CD13 |
| Comments/Corrections | |
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