Document Detail

Tyrosine sulphation is not required for microvillar expression of intestinal aminopeptidase N.
MedLine Citation:
PMID:  2902847     Owner:  NLM     Status:  MEDLINE    
The effect of 2,6-dichloro-4-nitrophenol (DCNP), an inhibitor of phenol sulphotransferases (EC 2.8.2.-), on the biosynthesis of aminopeptidase N (EC was studied in organ-cultured pig intestinal mucosal explants. At 50 microM DCNP did not affect protein synthesis but it decreased incorporation of [35S]sulphate into aminopeptidase N and other major microvillar hydrolases by 70-85% compared with controls, indicating an inhibition of their post-translational tyrosine sulphation. In labelling experiments with [35S]methionine from 0.5 to 5 h, DCNP was tested for its possible influence on synthesis, processing and microvillar expression of aminopeptidase N, but no effect on any of these parameters could be detected. It can therefore be concluded that tyrosine sulphation is not required (for instance as a sorting signal) for the targeting of newly synthesized enzymes to the microvillar membrane.
E M Danielsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  254     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-11-22     Completed Date:  1988-11-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  219-22     Citation Subset:  IM    
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
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MeSH Terms
Aminopeptidases / biosynthesis*
Antigens, CD13
Electrophoresis, Polyacrylamide Gel
Enzyme Induction / drug effects
Intestinal Mucosa / drug effects,  enzymology*
Microvilli / drug effects,  enzymology
Nitrophenols / pharmacology
Organ Culture Techniques
Protein Biosynthesis
Sulfur Radioisotopes
Tyrosine / metabolism*
Reg. No./Substance:
0/Nitrophenols; 0/Sulfur Radioisotopes; 55520-40-6/Tyrosine; 618-80-4/2,6-dichloro-4-nitrophenol; EC 3.4.11.-/Aminopeptidases; EC, CD13

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