| Tyrosine promotes oxidative stress in cerebral cortex of young rats. | |
| | |
MedLine Citation:
|
PMID: 18602789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Tyrosine accumulates in inborn errors of tyrosine catabolism, especially in tyrosinemia type II, where tyrosine levels are highly elevated in tissues and physiological fluids of affected patients. In tyrosinemia type II, high levels of tyrosine are correlated with eyes, skin and central nervous system disturbances. Considering that the mechanisms of brain damage in these disorders are poorly known, in the present study, we investigated whether oxidative stress is elicited by l-tyrosine in cerebral cortex homogenates of 14-day-old Wistar rats. The in vitro effect of 0.1-4.0mM l-tyrosine was studied on the following oxidative stress parameters: total radical-trapping antioxidant potential (TRAP), total antioxidant reactivity (TAR), ascorbic acid content, reduced glutathione (GSH) content, spontaneous chemiluminescence, thiobarbituric acid-reactive substances (TBA-RS), thiol-disulfide redox state (SH/SS ratio), protein carbonyl content, formation of DNA-protein cross-links, and the activities of the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glucose-6-phosphate dehydrogenase (G6PDH). TRAP, TAR, ascorbic acid content, SH/SS ratio and CAT activity were significantly diminished, while formation of DNA-protein cross-link was significantly enhanced by l-tyrosine in vitro. In contrast, l-tyrosine did not affect the other parameters of oxidative stress evaluated. These results indicate that l-tyrosine decreases enzymatic and non-enzymatic antioxidant defenses, changes the redox state and stimulates DNA damage in cerebral cortex of young rats in vitro. This suggests that oxidative stress may represent a pathophysiological mechanism in tyrosinemic patients, in which this amino acid accumulates. |
| | |
Authors:
|
Angela M Sgaravatti; Bethânia A Vargas; Bernardo R Zandoná; Kátia B Deckmann; Francieli J Rockenbach; Tarsila B Moraes; José M Monserrat; Mirian B Sgarbi; Carolina D Pederzolli; Angela T S Wyse; Clóvis M D Wannmacher; Moacir Wajner; Carlos Severo Dutra-Filho |
Related Documents
:
|
8924609 - Epoxidation of trans-4-hydroxy-2-nonenal by fatty acid hydroperoxides and hydrogen pero... 21271619 - Direct hydrogenation of nitroaromatics and one-pot amidation with carboxylic acids over... 18339249 - Protection of arsenic-induced testicular oxidative stress by arjunolic acid. 17743969 - Fast reactions of ascorbic acid and hydrogen peroxide in ice, a presumptive early envir... 10457989 - Effect of roseox on peroxidation of rat mitochondrial lipids. 21535429 - Usefulness of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin... 12021579 - Treatment of hyperhomocysteinemia with folic acid: effects on homocysteine levels, coag... 9134959 - Sequelae of parenteral domoic acid administration in rats: comparison of effects on dif... 4019919 - Retention and clearance of c-11 palmitic acid in ischemic and reperfused canine myocard... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-28 |
Journal Detail:
|
Title: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience Volume: 26 ISSN: 0736-5748 ISO Abbreviation: Int. J. Dev. Neurosci. Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-07-21 Completed Date: 2008-11-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8401784 Medline TA: Int J Dev Neurosci Country: England |
Other Details:
|
Languages: eng Pagination: 551-9 Citation Subset: IM |
Affiliation:
|
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Animals, Newborn Antioxidants / metabolism Ascorbic Acid / metabolism Catalase Cerebral Cortex / physiology* Dose-Response Relationship, Drug Glucosephosphate Dehydrogenase / metabolism Glutathione / metabolism Glutathione Peroxidase / metabolism Lipid Peroxidation / drug effects Oxidative Stress / drug effects* Rats Rats, Wistar Sulfhydryl Compounds / metabolism Superoxide Dismutase / metabolism Thiobarbituric Acid Reactive Substances Tyrosine / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Sulfhydryl Compounds; 0/Thiobarbituric Acid Reactive Substances; 50-81-7/Ascorbic Acid; 55520-40-6/Tyrosine; 70-18-8/Glutathione; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development and validation of RP-HPLC method for the determination of genotoxic alkyl benzenesulfona...
Next Document: Melanoma of the anorectal region The experience of the National Cancer Institute of Milano.