Document Detail

Tyrosine phosphorylation of a gap junction protein correlates with inhibition of cell-to-cell communication.
MedLine Citation:
PMID:  1963075     Owner:  NLM     Status:  MEDLINE    
Cell-to-cell communication is achieved by passage of small molecules through gap junction membrane channels. The expression of the transforming gene from Rous sarcoma virus, v-src, induces a rapid and dramatic reduction in cell-to-cell communication in cultured cells. To determine whether connexin43, a major gap junction protein expressed in fibroblasts, is a target for the v-src protein tyrosine kinase activity, we examined the phosphorylation state of connexin43 in cells expressing variants of src. Using an antipeptide serum that recognizes connexin43, we demonstrate that this protein is phosphorylated on serine and tyrosine residues in avian and mammalian cells expressing activated src proteins. Connexin43 from control cells and cells expressing nonactivated variants of the src protein was phosphorylated solely on serine residues. In lysates from v-src-transformed cells, all phosphorylated connexin43 molecules were cleared from the lysate by sequential immunoprecipitations using the phosphotyrosine antibodies, suggesting that each molecule of phosphorylated connexin43 contains both phosphoserine and phosphotyrosine. We have also examined junctional permeability in cells expressing src variants and find that loss of cell-to-cell communication correlates with tyrosine phosphorylation of connexin43.
A J Filson; R Azarnia; E C Beyer; W R Loewenstein; J S Brugge
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  1     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-04-04     Completed Date:  1991-04-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  661-8     Citation Subset:  IM    
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104.
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MeSH Terms
Cell Communication / physiology*
Cell Line, Transformed
Genes, src / physiology*
Intercellular Junctions / physiology*
Membrane Proteins / metabolism*
Molecular Weight
Precipitin Tests
Tyrosine / metabolism
Grant Support
Reg. No./Substance:
0/Connexins; 0/Membrane Proteins; 55520-40-6/Tyrosine

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