| Tyrosine phosphorylation of ACK in response to temperature shift-down, hyperosmotic shock, and epidermal growth factor stimulation. | |
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MedLine Citation:
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PMID: 8647288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mammalian Cdc42 protein regulates various kinds of cellular responses, including formation of filopodia, polarization of T cells, and cell cycle progression. A non-receptor tyrosine kinase ACK, which specifically binds to the GTP-bound form of Cdc42, was isolated as a putative target of Cdc42. Here we show the induction of tyrosine phosphorylation of ACK in response to temperature shift-down to 25 degrees C, and hypertonic shock, as well as stimulation with epidermal growth factor (EGF) in human embryonic kidney (HEK) 293 cells. The increased tyrosine phosphorylation level upon temperature shift-down was sustained for at least 60 min, whereas reversion of the temperature to 37 degrees C caused rapid tyrosine dephosphorylation to the initial level. The responses to EGF and the high osmolarity were transient. Furthermore, we observed association of ACK with an adaptor protein Grb2, which may suggest the involvement of Grb2 in EGF receptor-mediated tyrosine phosphorylation of ACK. |
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Authors:
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T Satoh; J Kato; K Nishida; Y Kaziro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 386 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-07-25 Completed Date: 1996-07-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 230-4 Citation Subset: IM |
Affiliation:
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Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing* Cell Line Epidermal Growth Factor / pharmacology* GRB2 Adaptor Protein Humans Osmotic Pressure Phosphorylation / drug effects Protein-Tyrosine Kinases / metabolism* Proteins / metabolism Temperature Tyrosine / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/GRB2 Adaptor Protein; 0/GRB2 protein, human; 0/Proteins; 55520-40-6/Tyrosine; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Protein-Tyrosine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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