Document Detail

Tyrosine phosphorylation of ACK in response to temperature shift-down, hyperosmotic shock, and epidermal growth factor stimulation.
MedLine Citation:
PMID:  8647288     Owner:  NLM     Status:  MEDLINE    
The mammalian Cdc42 protein regulates various kinds of cellular responses, including formation of filopodia, polarization of T cells, and cell cycle progression. A non-receptor tyrosine kinase ACK, which specifically binds to the GTP-bound form of Cdc42, was isolated as a putative target of Cdc42. Here we show the induction of tyrosine phosphorylation of ACK in response to temperature shift-down to 25 degrees C, and hypertonic shock, as well as stimulation with epidermal growth factor (EGF) in human embryonic kidney (HEK) 293 cells. The increased tyrosine phosphorylation level upon temperature shift-down was sustained for at least 60 min, whereas reversion of the temperature to 37 degrees C caused rapid tyrosine dephosphorylation to the initial level. The responses to EGF and the high osmolarity were transient. Furthermore, we observed association of ACK with an adaptor protein Grb2, which may suggest the involvement of Grb2 in EGF receptor-mediated tyrosine phosphorylation of ACK.
T Satoh; J Kato; K Nishida; Y Kaziro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  386     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  230-4     Citation Subset:  IM    
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
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MeSH Terms
Adaptor Proteins, Signal Transducing*
Cell Line
Epidermal Growth Factor / pharmacology*
GRB2 Adaptor Protein
Osmotic Pressure
Phosphorylation / drug effects
Protein-Tyrosine Kinases / metabolism*
Proteins / metabolism
Tyrosine / metabolism*
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/GRB2 Adaptor Protein; 0/GRB2 protein, human; 0/Proteins; 55520-40-6/Tyrosine; 62229-50-9/Epidermal Growth Factor; EC Kinases

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