Document Detail

Tyrosine kinase inhibitors suppress endotoxin- and IL-1 beta-induced NO synthesis in aortic smooth muscle cells.
MedLine Citation:
PMID:  8214107     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) formation via the expression of an endotoxin- and cytokine-inducible NO synthase (iNOS) within the vascular smooth muscle is thought to be responsible for the cardiovascular collapse that occurs during septic shock and antitumor therapy with cytokines. Because the molecular mechanisms that underlie induction of iNOS are still unclear and because tyrosine kinases are implicated in interleukin-1 beta (IL-1 beta)-induced prostaglandin synthesis in mesangial cells and in NO generation by an insulinoma cell line, we investigated the influence of tyrosine kinase inhibitors on iNOS induction in cultured rat aortic smooth muscle cells (RASMC). The production of biologically active NO was demonstrated by L-arginine-dependent guanosine 3',5'-cyclic monophosphate (cGMP) accumulation after a 3-h exposure to either IL-1 beta or lipopolysaccharide (LPS). Pretreatment of RASMC for 30 min with the tyrosine kinase inhibitor genistein prevented both IL-1 beta- and LPS-elicited cGMP accumulation in a concentration-dependent manner. Geldanamycin, a chemically different tyrosine kinase inhibitor, also blocked cGMP formation in response to both LPS and IL-1 beta at nanomolar concentrations. Genistein and geldanamycin inhibited cGMP accumulation even when added 90 min after LPS exposure, but no inhibition was observed when they were included at later time points (120-180 min), suggesting that the inhibitors had no direct effect on iNOS activity after its induction. Formation of cGMP in response to sodium nitroprusside and to NO released from bovine aortic endothelial cells remained virtually unaffected by genistein and geldanamycin.(ABSTRACT TRUNCATED AT 250 WORDS)
N Marczin; A Papapetropoulos; J D Catravas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  265     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-11-22     Completed Date:  1993-11-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1014-8     Citation Subset:  IM    
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta 30912-2300.
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MeSH Terms
Aorta / cytology,  metabolism*
Cyclic GMP / metabolism
Endotoxins / pharmacology*
Interleukin-1 / pharmacology*
Isoflavones / pharmacology
Lactams, Macrocyclic
Lipopolysaccharides / pharmacology
Muscle, Smooth, Vascular / cytology,  metabolism*
Nitric Oxide / biosynthesis*
Protein-Tyrosine Kinases / antagonists & inhibitors*
Quinones / pharmacology
Grant Support
Reg. No./Substance:
0/Benzoquinones; 0/Endotoxins; 0/Interleukin-1; 0/Isoflavones; 0/Lactams, Macrocyclic; 0/Lipopolysaccharides; 0/Quinones; 10102-43-9/Nitric Oxide; 30562-34-6/geldanamycin; 446-72-0/Genistein; 7665-99-8/Cyclic GMP; EC Kinases

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