Document Detail


Tyramine amplification technique in routine immunohistochemistry.
MedLine Citation:
PMID:  9358847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Signal amplification in immunohistochemistry via binding of biotinylated tyramine to proteins near the site of peroxidase-labeled antibodies is a promising new technique, but studies investigating a wide range of markers are lacking. The tyramine amplification technique (TAT) was investigated on 85 antibodies using a simple and fast protocol, and TAT results were compared to those obtained with conventional immunohistochemistry. Using TAT, most of the markers could be 5- to 50-fold further diluted and still showed identical staining results compared with standard stainings (maximal 500-fold). However, the variable reactivity of the different markers with TAT underlines the need for individual testing of every antibody to determine the optimal dilution. Some antibodies against cell adhesion molecules could be demonstrated for the first time in archival, formalin-fixed tissue sections. TAT, if carefully evaluated, offers a revolutionary improvement for modern immunostaining, either to increase sensitivity or primary antibody dilutions (cost reduction). From a methodological point of view, immunohistochemistry has not reached its limits by far and TAT is an important progressive step in this developmental process.
Authors:
R von Wasielewski; M Mengel; S Gignac; L Wilkens; M Werner; A Georgii
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  45     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-11     Completed Date:  1997-12-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1455-9     Citation Subset:  IM    
Affiliation:
Pathologisches Institut, Medizinischen Hochschule Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Biotin / analogs & derivatives*
Evaluation Studies as Topic
Immunohistochemistry / methods*
Sensitivity and Specificity
Tyrosine / analogs & derivatives*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 55520-40-6/Tyrosine; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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