Document Detail


[Typing of enteric adenoviruses in feces of infants with diarrhea]
MedLine Citation:
PMID:  11498698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study the infection condition of adenovirus types in infants with diarrhea in Shenzhen. METHODS: 114 fecal samples from diarrhea infants were tested by PCR. The amplified products were analyzed by Taq I and Rsa I endonucleases. RESULTS: The positive rate of adenovirus DNA in fecal samples was 14% (16/114). The amplified products typing procedure started with Taq I endonuclease to identify the type of adenovirus, 11 cleaved by Taq I yielded two fragments of 191 bp and 110 bp, the positive rate of enteric adenovirus was 9.65% (11/114). Followed by Rsa I cleavage, two amplified products yielded 256 bp and 45 bp fragments (Ad40), the positive rate of Ad40 was 1. 75% (2/114), nine amplified products yielded 211 bp and 90 bp fragments (Ad4l), the positive rate of Ad41 was 7.89%(9/114). CONCLUSIONS: The positive rates of the adenovirus types 40 and 41 were 9.65% in feces of infants with diarrhea, and that of the other types of adenovirus was 4.35%.
Authors:
Y He; H Yang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology     Volume:  14     ISSN:  1003-9279     ISO Abbreviation:  Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-08-10     Completed Date:  2003-11-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9602873     Medline TA:  Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi     Country:  China    
Other Details:
Languages:  eng     Pagination:  278-80     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Shenzhen Health and Anti-epidemic Station, Shenzhen 5l8020, China.
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MeSH Terms
Descriptor/Qualifier:
Adenovirus Infections, Human / epidemiology*
Adenoviruses, Human / classification,  genetics,  isolation & purification*
China / epidemiology
DNA, Viral / analysis
Diarrhea, Infantile / virology*
Feces / virology*
Humans
Infant
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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